木鳖子提取物对小鼠急性毒性实验研究

Experimental Study on Acute Toxicity of Momordica Cochinchinensis in Mice

目的:研究木鳖子提取物对小鼠急性毒性的影响,进行木鳖子的急性毒性观察与评价,为安全用药提供依据。方法:按照小鼠急性毒性经典实验方法,对木鳖子提取物不同给药途径的小鼠急性毒性实验进行比较研究,以概率单位法(Bliss法)计算木鳖子口服和腹腔注射时半数致死量(LD_(50)),给药后连续观察小鼠状态7 d,实验结束后处死小鼠,观察重要脏器是否存在病理改变。结果:小鼠口服木鳖子提取物半数致死量LD_(50)=4.03 g·kg^(-1),95%置信区间(CI))为3.29~4.92 g·kg^(-1);小鼠腹腔注射木鳖子提取物半数致死量LD_(50)=146.17 mg·kg^(-1),95%CI为118.92~177.31 mg·kg^(-1)。结论:实验表明木鳖子药材具有一定的毒性,与文献和药典报道相符。通过木鳖子小鼠急性毒性实验研究,有利于进行木鳖子的毒性研究与评价,为临床安全用药提供实验依据。

Objective： To study the acute toxicity of Momordica Cochinchinensis in mice, observe and evaluate the acute toxicity of Momordica Cochinchinensis herbs in order to provide basis for the safe and reasonable clinical medication. Methods： According to the classic experiment methods, the acute toxicity of Momordica Cochinchinensis in mice was compared with different administration methods, and the LD50 was calculated by the Bliss method with the continuous administration and observation for 7 d. At the end of the trial, the pathologic changes of the relevant organs were also observed. Results： The LD50 of Momordica Cochinchinensis in mice with oral administration was 4.03g·kg^-1, and the 95 % confidence interval was 3.29-4.92 g·kg^-1. The LDsoof Momordica Cochinchinensis in mice with intraperitoneal injection was 146.17 mg·kg^-1 , and the 95% confidence interval was 118.92-177.31 mg·kg^-1. Conclusion： The Momordica Cochinchinensis extract is poisonous, which is consistent with the literatures and pharmacopoeia. By comparing the acute toxicity with different administration methods of Momordica Cochinchinensis in mice, it is beneficial to the evaluation of acute toxicity of Momordica Cochinchinensis to provide basis for the reasonable medication in clinics.