利用表面增强拉曼光谱技术检测硫酸阿托品

Detection of Atropine Sulfate by Surface Enhanced Raman Spectroscopy

目的:建立硫酸阿托品的表面增强拉曼光谱(SERS)技术检测方法。方法:通过理论计算与试验检测相结合,对硫酸阿托品分子的拉曼峰进行归属,并选取1 002 cm^(-1)拉曼峰作为特征峰,开展定量分析。结果:硫酸阿托品水溶液中的检测限约为0.5μg"ml^(-1);1 002 cm^(-1)处特征峰强度随水溶液浓度变化关系的线性范围在1~8μg·ml^(-1),r=0.983 9;平均回收率为103.3%,RSD为4.5%(n=9)。同时,对该方法的批次间稳定性进行了测试,相对标准偏差为5.7%。另外,对含有硫酸阿托品的中药苍术掺杂样品进行检测,依然能检测到1 002 cm^(-1)处的特征峰。结论:该方法具有快速、准确、无损、操作简便等优点,在硫酸阿托品检测方面具有很好的应用潜力。

Objective： To establish a surface enhanced Raman spectroscopy （SERS） technique for the determination of atropine sulfate. Methods： The Raman peaks of atropine sulfate molecules were classified by theoretical calculations and experimental tests, and the 1 002 cm^-1 Raman peaks were selected as the characteristic peaks for the quantitative analysis. Results： The detection limit of atropine sulfate in aqueous solution was below 0.5μg·ml^-1. The relationship between the intensity of characteristic peaks at 1 002 cm^-1 and the concentration of aqueous solution was linear within the range of 1-8 μg·ml^-1 with the linear correlation coefficient r of 0.983.9. The recovery rates of 2, 5 and 7μg·ml^-1 were measured, which were 97.1% -109.8%. The average recovery was 103.3% , and the RSD was 4.5% （ n = 9）. At the same time, the stability of the method among the batches was tested, and the relative standard deviation was 5.7 %. In addition, the atractylodes rhizome samples containing atropine sulfate were detected, and the characteristic peaks still could be detected at 1 002 cm^-1. Conclusion： The method is rapid, accurate and nondestructive with easy operation, which can be used for the detection of atropine sulfate.