戊己汤结合针刺对慢传输型便秘大鼠碳末推进率、PI3K mRNA及NOS mRNA表达的影响

The Influence of Wu-Ji Decoction Combined with Electroacupuncture on the Relevant Indexes of Rats with Slow Transit Constipation

为了探讨戊己汤灌胃结合针刺对慢传输型便秘（STC）模型大鼠碳末推进率、PI3K mRNA及NOS mRNA表达的影响,将57只Wistar大鼠适应性喂养1周后随机分为空白组、模型组、实验组3组,每组1 9只.除空白组外所有大鼠均建立STC大鼠模型.造模成功后,实验组以电针针刺双侧“天枢”穴15 min,1次/d,并采用戊己汤（生药4.8 g/kg·d）灌胃,1次/d,连续治疗15 d,模型组使用等量生理盐水灌胃,1次/d,连续15 d,末次灌胃后,禁食24 h.观察实验大鼠的肠道碳末推进率、PI3K mRNA及NOS mRNA的表达.结果显示,模型组碳末推进率低于空白组（P＜0.01）,实验组碳末推进率高于空白组和模型组（P ＜0.05,P＜0.01）.模型组PI3K mRNA表达低于空白组,NOS mRNA表达高于空白组,有显著性差异（P＜0.05）;实验组PI3K mRNA表达高于模型组,NOS mRNA表达低于模型组,有显著性差异（P＜0.05）.结果表明,戊己汤灌胃结合针刺对STC大鼠肠道传输有明显改善作用.

This study was to explore the influence of Wu-Ji decoction gavage combined with electroacu- puncture on the relevent indexes of model rats with slow transit constipation （STC） including carbon parti- cles propelling rate（CPPR）,PI3K mRNA and NOS mRNA expression, randomly divided 57 Wistar rats af- ter accommodative feeding for 1 week into blank group,model group,and experimental group,19 cases for each;the latter two groups were established as STC model;then experimental group performed electroacu- puncture at bi-side Tianshu point for 15 rain, 1 time/day,gavaged with Wu-Ji decoction（raw drugs 4.8 g/kg d）, 1 time/day, for continuously 15 days, meanwhile the model group received same volume of normal sa- line as Wu-Ji decoction in experimental group, 1 time/day, for 15 days;after the last time performed abso- lute diet fast for 24 hrs;observed rats＂ following indexes as CPPR,the expression of PI3K mRNA and NOS mRNA.As results,in CPPR model group was lower than blank group（ P 〈0.01） , experimental group was higher than blank group and model group （P 〈0.05 and P 〈0.01）~in PI3K mRNA expression model group was lower than blank group and experimental group was higher than model group（ P 〈0.05）;in NOS mRNA expression model group was higher than blank group, trial group was lower than model group,there was significant difference （ P〈0.05）.Results show that Wu-Ji decoction combined with elec- troacupuncture has significant role for improving STC rat＇s intestinal transition.