基于灰色关联法研究胆矾中元素对A549细胞的体外抑制作用

Study on the Inhibition Lung Cancer Cell A549 with Traditional Chinese Mineral Drugs Danfan in Vitro Basing on the Grey Correlation Method

目的探讨胆矾水煎液的元素组成,及其中与体外A549细胞抑制率关联度最大的元素。方法通过ICP-MS对胆矾水煎液中的元素组成进行分析;用MTT法测得不同浓度下胆矾水煎液对A549细胞的抑制率;通过灰色关联度软件分析与抑制率关联系数最大的元素;用流式细胞术验证促进肺肿瘤细胞凋亡的元素种类。结果胆矾水煎液的主要元素组成为Cu、Zn、Fe、Cd、Mn、Al、Na、Ca等,其中Cu与A549细胞的抑制率关联系数最大,流式细胞研究进一步验证了促进肺癌细胞凋亡的元素种类。结论胆矾水煎液中铜离子与抑制肿瘤生长的关系较大。

OBJECTIVE To investigate the component elements in the water decoction of traditional Chinese mineral drugs Danfan, and the most important element associated with the inhibition rate of A549 cells in vitro. METHODS The total elements in the water decoction of traditional Chinese mineral drugs Danfan were analyzed by ICP-MS. The inhibition ratio of different elements of water decoction of Danfan to the A549 cell were detected by MTT assay. Then the Grey relational grade between the inhibition ratio and elements was analyzed, and flow cytometry was used to verify the results that which element was promoting apoptosis of lung tumor cells. RESULTS The main elements of the water decoction of Danfan were Cu, Zn, Fe, Cd, Mn, Al, Na, Ca and so on. The correlation coefficient between Cu and A549 cell inhibition rate was the largest. The flow cytometry research further validated that which elements promoting apoptosis of lung cancer cells. CONCLUSION The copper element in Danfan is an important element in the tumor cell apoptosis activity.