川芎嗪对高糖诱导的人视网膜血管内皮细胞增殖的影响

Effects of Ligustrazine on proliferation of human retinal vascular endothelial cells induced by high glucose

目的观察川芎嗪对高糖诱导的人视网膜血管内皮细胞(HRCECs)增殖的影响。方法将HRCECs细胞分为对照(生理盐水)组、模型(25 mmol/L葡萄糖)组和川芎嗪低、中、高浓度(50、100、200μmol/L)组,培养48 h后,MTT细胞毒实验检测细胞增殖变化,流式细胞技术检测细胞周期,ELISA法检测血管内皮生长因子(VEGF)表达。结果与对照组比较,高糖对HRCECs细胞增殖、分裂(M)期比例、上清VEGF浓度均具有显著促进作用(P<0.05、0.01);与模型组比较,低、中、高浓度川芎嗪对高糖诱导的HRCECs细胞增殖、分裂(M)期比例、上清VEGF浓度均发挥显著抑制作用(P<0.05、0.01),且呈浓度相关性。结论川芎嗪可能通过抑制高糖诱导的HRCECs细胞VEGF高表达,阻滞细胞周期,发挥抑制HRCECs细胞增殖的作用。

Objective To observe the effects of Ligustrazine on proliferation of human retinal vascular endothelial cells （HRCECs） induced by high glucose. Methods HRCECs cells were divided into control （saline） group, model （25 mmol/L glucose） group, ligustrazine low, medium, high concentration （50, 100 and 200 mol/L） group, cultured for 48 h. Cell proliferation was detected by MTT cytotoxicity assay. Flow cytometry was used to detect cell cycle, and the expression of VEGF was detected by EL1SA analysis. Results Compared with control group, HRCECs cell proliferation, cell division （M） phase ratio and supematant of VEGF concentration in model group increased significantly （P 〈 0.05, 0.01）. Compared with model group, cell proliferation, M phase ratio and supematant of VEGF in Ligustrazine low, medium and high concentration groups decreased significantly （P 〈 0.05, 0.01）, and showed a concentration dependence. Conclusion Ligustrazine can inhibit the expression of VEGF in HRCECs cells induced by high glucose to block cell cycle and inhibit the proliferation of HRCECs cells.