降植烷诱导小鼠系统性红斑狼疮模型研究

Experimental systemic lupus erythematosus mouse model induced by pristane

目的建立降植烷诱导的系统性红斑狼疮(SLE)小鼠模型,并对模型进行全面验证。方法 6~8周龄雌性BALB/c小鼠随机分为两组,模型组单次ip降植烷0.5 m L,对照组单次ip生理盐水0.5 m L。注射前及注射后2、3、4、5、6个月ELISA法检测血清中抗SLE抗体(anti-SLE)、抗双链DNA抗体(anti-ds DNA);注射前及注射后每月1次采用目测尿蛋白试纸测定小鼠尿蛋白;6个月处死动物,观察肾脏HE染色及直接免疫荧光染色后镜下变化。结果小鼠ip降植烷2个月后,其自身抗体(anti-ds DNA、anti-SLE)浓度显著高于对照组(P<0.05、0.01),且6个月内抗体浓度逐渐升高;6个月时,73%模型组小鼠出现++++尿蛋白;肾脏HE切片显示模型组小鼠肾脏出现肾小球肿胀、炎症细胞浸润等典型的肾病理改变,直接免疫荧光染色见模型组小鼠肾小球毛细血管存在免疫复合物沉积,对照组小鼠肾脏组织未见改变。结论降植烷成功诱导SLE动物模型。

Objective To establish the systemic lupus erythematosus （SLE） mouse model through pristaneip injection and validate the model comprehensively. Methods Female BALB/c mice of 6--8 weeks were randomly divided into two groups. Animals in model group were injected .with 0.5 mL pristane by ip injection while in control group with 0.5 mL normal saline, Anti-systemic lupus erythematosus antibodies （anti-SLE） and anti-double strand DNA antibodies （anti-dsDNA） were checked before injection and monthly thereafter. Proteinuria was detected before injection and every month thereafter. All mice were bled to death 6 months after injection. Kidneys were excised to observe the histopathologic evidence of glomerulonephritis. Results The concentration of anti-dsDNA and anti-SLE antibody in sera was higher of model group than that of control group two months after pristane injection, and the concentration of antibody gradually increased within 6 months. At the sixth months, the protein concentration of urine in most model group mice was ＋＋＋＋. The histopathology and imunoflorescence of kidney sections indicated typical evidence of glomerulonephritis in model group. Conclusion The murine lupus model can be successfully established in female BALB/c mouse with a single ip injection of 0.5mL ofpristane.