Krüpple样因子5在成牙本质细胞中的表达及临床意义

Expression pattern of Krüpple-like factor 5 in odontoblasts

目的 :研究转录因子Krüpple样因子5(Krüpple-like factor 5,KLF5)在人牙髓-牙本质复合体中的表达,探讨其在成牙本质细胞分化及牙本质形成过程中的作用。方法:应用免疫组织化学方法检测KLF5在人牙髓-牙本质复合体中及牙髓细胞矿化诱导过程中的表达,Western印迹法检测KLF5在成牙本质细胞分化过程中的表达变化。采用SPSS17.0软件包对实验结果进行单因素方差分析和Tukey检验。结果 :在正常及龋坏牙中,KLF5表达于人成牙本质细胞和成牙本质样细胞,而在牙本质及前期牙本质中无表达。细胞免疫组织化学结果显示,在矿化诱导0、7、14 d的人牙髓细胞中,KLF5在细胞核中呈强阳性表达。Western印迹结果显示,随着矿化诱导时间的延长,KLF5蛋白表达量逐渐增加。诱导14~21 d时,KLF5表达量增加最显著。结论:KLF5可能参与成牙本质细胞分化及牙本质的形成过程。

PURPOSE： To investigate the expression of Krüpple-like factor 5 （KLF5） in human pulp-dentin complex and the function of KLF5 in differentiation and dentinogenesis of odontoblasts. METHODS： Immunohistochemistry staining was used to investigate the expression of KLF5 in human pulp-dentin complex and dental pulp cells. Western blot was used to analyze the expression patterns of KLF5 during dental pulp cell differentiation. Significant differences between groups were evaluated using one-way ANOVA and Tukey＇s test with SPSS17.0 software package. RESULTS： Immunohistochemical staining showed KLF5 was positively expressed in the odontoblasts and odontoblast-like cells, but not in dentin and predentin in both normal and carious teeth. Moreover, during mineralization of human dental pulp cells, KLF5 was primarily localized in the nuclei of dental pulp cells. Western blot showed KLF5 was significantly up-regulated during odontoblast differentiation. CONCLUSIONS： KLF5 may play an important role in odontoblast differentiation and dentin formation.