3种树脂水门汀对人牙龈成纤维细胞的体外细胞毒性

Cytotoxicity of 3 resin cements to human gingival fibroblasts

目的:比较3种不同树脂水门汀对人牙龈成纤维细胞(human gingival fibroblasts,HGFs)的体外细胞毒性。方法:采用CCK-8法检测3种树脂水门汀(Panavia F、Rely XTM Unicem和Multilink Speed)48 h不同浓度(50%和100%)浸提液培养HGFs(ATCC,美国)24 h、72 h和120 h后的细胞相对增殖率(relative growth rate,RGR),判断其细胞毒性。采用SPSS 20.0软件包分析不同树脂水门汀对HGFs的RGR是否存在显著影响。结果:3种树脂水门汀不同浓度(50%和100%)浸提液培养HGFs的RGR为60.25%~100.02%,细胞毒性分级为0~2级,各组之间差异无显著性(P>0.05);固化过程中未涂布防氧化剂的Panavia F浸提液培养HGFs的RGR为10.67%~66.78%,细胞毒性分级为2~4级,与其他实验组相比,有显著差异(P<0.05)。结论:3种树脂水门汀对体外培养的HGFs无细胞毒性,固化过程中未涂布防氧化剂的Panavia F对体外培养的HGFs有中度细胞毒性。

PURPOSE： To study the cytotoxicity of 3 resin cements to human gingival fibroblasts （HGFs）. METHODS： Three resin cements （Panavia F, RelyXTM Unicem and Multilink Speed） test samples were immersed and incubated in the culture medium for 48 h at 37℃. Cultured HGFs were exposed to two concentrations （50% and 100%） of material elutes for 24 h, 72 h and 120 h. The proliferation rate was evaluated using CCK-8 assay. The data were statistically analyzed by one-way analysis of variance using SPSS 20.0 software package. RESULTS： Relative growth rate（RGR） of all experimental groups ranged from 10.67% to 100.02%, the cytotoxicity grade of all groups was 0 to 4. There was no significant difference in the RGR among 3 resin cements, but the experimental group of Panavia F （uncovered with antioxidant） showed significantly lower RGR than other experimental groups. CONCLUSIONS： Panavia F, RelyXTM Unicem and Multilink Speed exhibit no cytotoxicity to HGFs, Panavia F（uncovered with antioxidant） shows moderate cytotoxicity.