龙头竹组培快繁技术研究

Study on Tissue Culture and Rapid Propagation Technology of Bambusa vulgaris Schrader ex Wendland

试验研究了龙头竹不同外植体类型、不同消毒药剂及消毒时间对组培效果的影响,以及不同炼苗时间、不同育苗基质、不同肥料种类及施肥方式对组培苗移栽成活率和发笋率的影响。结果表明:龙头竹播种苗秆芽是最适合的组培外植体,外植体消毒以0.1%氯化汞、消毒7 min效果最好;组培苗移栽后炼苗9 d,育苗基质宜采用20%蛭石和80%珍珠岩混合基质,移栽15 d后叶面施用0.3%氮磷钾复合肥组培苗生长状况最好。

The effects of different explant types,different disinfectants and disinfection time on tissue culture of Bambusa vulgaris Schrader ex Wendland were studied experimentally. The effect of different cultivation time,different seedling substrates,different types of fertilizers and fertilization modes on the survival rate and new shoot rate of the transplanted bamboo tissue culture seedlings were also carried out in this study. The result indicated that the bud on the culm of B. vulgaris seedlings was the most suitable explant for tissue culture,and the best disinfection effect was 7 min with 0. 1% mercuric chloride. After transplanting,the tissue culture seedlings were in the best growth through being hardened for 9 days in the mixed substrate of 20% vermiculite and 80% pearlite,and being fertilized with 0. 3% NPK compound on leaves after transplanting for 15 days.