自贡市2007-2010年鼠伤寒沙门菌感染的脉冲场凝胶电泳分析

Analysis of Salmonella typhimurium infection by pulsed field gel electrophoresis in Zigong City,2007-2010

目的回顾性分析自贡市2007-2010年分离到的沙门菌菌株,评估分子分型技术在食源性传染病暴发识别和处置中的价值。方法对自贡市2007-2010年从食品和腹泻患者中分离的沙门菌进行脉冲场凝胶电泳(PFGE)实验,用Bio Numerics软件分析菌株之间的相似度。结果 55株沙门菌中,来源于2007-2008年的10个腹泻患者粪便和2008年的15个皮蛋中的25株鼠伤寒沙门菌菌株PFGE带型相似度为100%,均为P9带型。结论感染鼠伤寒沙门菌的皮蛋是导致自贡市2007-2008年10名患者腹泻暴发的污染源,为快速准确识别食源性疾病暴发并及时溯源、控制,应在食品安全监测中普及PFGE等分子分型技术。

Objective To retrospectively analyze Salmonella strains isolated in Zigong City during 2007-2010, and to evaluate the value of molecular typing technique in identification and disposal of outbreaks of foodborne diseases. Methods Salmonella strains isolated from food and diarrhea patients in Zigong City during 2007-2010 were analyzed by pulsed field gel electrophoresis （PFGE）, and BioNumerics software was used to analyze the genetic similarity index of the Salmonella strains. Results According to the PFGE patterns, 25 of the 55 Salmonella typhimurium strains isolated from stool samples of 10 diarrhea patients in 2007-2008 and 15 preserved eggs in 2008 were classified into type P9, and their genetic similarity was 100%. Conclusions Eggs contaminated by Salmonella typhimurium were the pollution sources that caused the outbreaks of diarrhea in 10 patients in Zigong City during 2007-2008. To quickly and accurately identify foodborne disease outbreaks and timely control and trace the outbreaks, PFGE and other molecular typing techniques should be widely used in food safety monitoring.