Wnt5a和ROR1在骨肉瘤中的表达及临床意义

Expression of Wnt5a and ROR1 in osteosarcoma and their clinical significance

目的探讨Wnt5a和受体酪氨酸激酶样孤核受体1(ROR1)在骨肉瘤组织中的表达及其临床意义。方法采用免疫组织化学SP法检测35例骨肉瘤和15例骨软骨瘤中Wnt5a及ROR1的表达情况,并分析两者表达与临床病理特征及预后的关系。结果 Wnt5a和ROR1在骨肉瘤组织中的阳性表达率分别为62.9%(22/35)和57.1%(20/35),明显高于骨软骨瘤组织的13.3%(2/15)和6.7%(1/15),差异均有统计学意义(P<0.05)。在骨肉瘤组织中,Wnt5a和ROR1的表达与Enneking分期和远处转移密切相关(P<0.05)。Wnt5a与ROR1的表达呈正相关(r=0.888,P<0.001)。Kaplan-Meier生存分析显示,Wnt5a和ROR1阳性表达者的中位生存时间分别为23个月和27个月,明显短于阴性表达者的41个月和42个月(P<0.05)。结论 Wnt5a和ROR1在骨肉瘤中呈高表达,其阳性表达可能与骨肉瘤的发生、发展密切相关,联合检测Wnt5a、ROR1对于判断骨肉瘤的进展及预后具有重要意义。

Objective To investigate the expression and clinical significance of Wnt5a and receptor tyrosine kinase-like orphan receptor 1（ROR1） proteins in human osteosarcomatous tissues.Methods The expression of Wnt5a and ROR1 in 35 specimens of osteosarcoma tissues and 15 specimens of osteochondroma tissues was detected by immunohistochemical SP method.The correlation of Wnt5a and ROR1 expression with clinicopathological characteristics and prognosis of the patients with osteosarcoma was analyzed.Results The positive expression rates of Wnt5a and ROR1 in osteosarcoma tissues were 62.9%（22/35） and 57.1%（20/35）,significantly higher than 13.3%（2/15） and 6.7%（1/15） of osteochondroma tissues,and the difference was statistically significant（P 0.05）.The expression of Wnt5a and ROR1 was positively related to Enneking clinica1 stages and metastasis（P 0.05）.The expression of Wnt5a was positively correlated with ROR1 protein（r = 0.888,P 0.001）.Kaplan-Meier analysis showed that the median overall survival of osteosarcoma patients with positive Wnt5a and ROR1 expression were 23 months and 27 months,significantly shorter than 41 months and 42 months of negative expression patients（P 0.05）.Conclusion Wnt5a and ROR1 were highly expressed in osteosarcoma and associated with the development and progression of osteosarcoma.Combined detection of Wnt5a and ROR1 is of great significance in judging the progress and prognosis of osteosarcoma.