瑞芬太尼对人肺腺癌细胞转移能力和白细胞介素-7受体表达的影响

Effect of remifentanii on metastasis of human lung adenocarcinoma cells and expression of interleu-kin-7 receptors

目的评价瑞芬太尼对人肺腺癌细胞转移能力和白细胞介素-7受体（IL-7R）表达的影响。 方法人肺腺癌细胞系A549细胞，以2×105/ml的密度接种于24孔培养板中，72个培养孔采用随机数字表法分为4组（n＝18）：瑞芬太尼2、4、6 ng/ml组（R2组、R4组和R6组）加入相应终浓度的瑞芬太尼孵育4 h，正常对照组（C组）加入等容量生理盐水。洗除药物后再培养或孵育24 h收集细胞，采用Transwell法检测侵袭细胞数，采用细胞划痕法检测细胞迁移率，采用Western blot法检测IL-7R的表达。 结果与C组比较，R2组、R4组和R6组侵袭细胞数和细胞迁移率降低，R4组和R6组IL-7R表达下调（P〈0.05）；与R2组比较，R4组和R6组侵袭细胞数和细胞迁移率降低，IL-7R表达下调（P〈0.05）；与R4组比较，R6组侵袭细胞数和细胞迁移率降低，IL-7R表达下调（P〈0.05）。 结论瑞芬太尼可抑制人肺腺癌细胞转移能力和下调IL-7R表达。

Objective To evaluate the effects of remifentanil on the metastasis of human lung ade- nocarcinoma cells and expression of interleukin-7 receptor （IL-TR）. Methods Human adenocarcinoma cell line A549 cells were seeded in 24-well plates at a density of 2x 105 cells/ml （72 wells）. The cells were divided into 4 groups （n= 18 each） using a random number table： normal control group （group C） and remifentanil 2, 4 and 6 ng/ml groups （group R2, group R4, group R6 ）. In R2, R4 and R6 groups, remifentanil at the final concentrations of 2, 4 and 6 ng/ml was added, respectively, and the cells were in- cubated for 4 h. The equal volume of normal saline was given instead of remifentanil in group C. The cells were collected at 24 h after the following incubation. The invasion of cells was determined by Transwell in- vasion assay, and the invaded cells were counted. The migration of cells was determined by cell scratch test, and cell migration rates were calculated. The expression of IL-7R in cells was detected by Western blot. Results Compared with group C, the number of invaded cells and cell migration rates were signifi- cantly decreased in R2 , R4 and R6 groups, and the expression of IL-7R was down-regulated in R4 and R6 groups （P〈0.05）. Compared with group R2, the number of invaded cells and cell migration rates were significantly decreased, and the expression of IL-7R was down-regulated in R and R6 groups （ P〈0.05）. Compared with group R4 , the number of invaded cells and cell migration rates were significantly decreased, and the expression of IL-7R was down-regulated in group R6 （ P〈 0.05）. Conclusion Remifentanil can inhibit metastasis of human lung adcnocarcinoma cells and down-regulate the expression of IL-7R.