西柏三烯-4,6-二醇降解产香菌培养基条件优化

Optimization of medium for cembratriene-4,6-diol degradation bacteria

为提高新鞘氨醇杆菌(Novosphingobium panipatense YII-3)对西柏三烯-4,6-二醇的降解效率,利用响应面法对发酵培养基的组成进行了优化。结果表明:(1)通过单因素实验确定了最佳碳源及其浓度、氮源及其浓度、初始pH和培养温度。(2)以上述4个单因素为考察因子设计了4因素3水平的Box-Behnken响应面分析实验,得到的最佳培养基组成为麦芽糖0.66 g/L、酵母粉0.91 g/L、pH=9和培养温度35℃。(3)该条件下培养的新鞘氨醇杆菌对西柏三烯-4,6-二醇的降解率从最初的64.58%增加到84.71%,与优化前相比提高了20.13百分点。Box-Behnken响应面分析实验用于新鞘氨醇杆菌的培养基优化是可行的,且数学模型的预测值与实验观察值相符。

In order to improve the degradation rate of cembratriene-4,6-diol, the culture medium for Novosphingobium panipatense YII-3 was optimized by conducting response surface analysis. The results showed that： 1） The optimal carbon source and its concentration, nitrogen source and its concentration, initial pH and cultural temperature for Novosphingobium panipatense YII-3 were confirmed by single factor experiment. 2） The said 4 factors were selected as the response factors, the Box-Behnken response surface analysis of the 4 factors at 3 levels was designed with the degradation rate as the response value. The optimal constituents of the culture medium were determined as maltose 0.66 g/L, yeast extract 0.91 g/L, initial pH 9, and cultural temperature at 35 ℃. 3） Under that condition, the degradation rate increased from 64.58% to 84.71% （or increased by 20.13 percentage points） after optimization. The observed experimental values agreed with the predictive values of mathematic models, and Box-Behnken response surface analysis is suitable for optimizing the culture medium of Novosphingobium panipatense.