实验性自身免疫性葡萄膜炎模型中肝脏免疫系统紊乱的研究（英文）

A Disorder of The Liver Immune System in Experimental Autoimmune Uveitis

葡萄膜炎是一种反复发作的炎症性疾病,可导致免疫系统功能障碍和多器官损伤.然而,葡萄膜炎是否导致肝功能损害尚不十分清楚.本文通过运用流式分析技术和激光共聚焦成像技术,研究了实验性自身免疫葡萄膜炎模型的肝脏病理和功能变化.结果显示肝损伤可出现在葡萄膜炎的炎症后期并与眼损伤程度相关.并且CD3^+CD4^+T细胞、CD3^-NK1.1^+DX5^-NK细胞、和CD11b~^+F4/80^-ly6c^+细胞在感染的眼睛和肝脏中增加.将CD3^+CD4^+T细胞回输给炎症的小鼠后,眼睛和肝脏的病理损伤加重.此外,在炎症的小鼠中可见血管扩张,大量淋巴细胞浸润到炎症的眼和肝脏的血管周围.总之,我们的研究结果提示,肝损伤可以发生在小鼠葡萄膜炎模型中,这种损伤可能与通过外周循环浸润到肝脏的CD3^+CD4^+T细胞有关.

Uveitis is a recurrent inflammatory disease that can lead to immune system dysfunction and multiple organ injuries.However, whether uveitis causes liver functional damage is still unclear. Herein, by using flow cytometry and confocal, we investigated the pathological and functional changes of liver in an experimental autoimmune model of uveitis（EAU）. Hepatic damage was observed at the inflammatory stage of uveitis and was associated with severity of eye damage. Moreover, the expression of CD3~＋CD4~＋ T cells,CD3^-NK1.1~＋ DX5^-NK cells, and CD11 b~＋F4/80^-Ly6c~＋ cells were increased in the inflamed eye and liver. Furthermore, the pathological damage of EAU and hepatic impairment were aggravated after transferring CD3~＋CD4~＋T cells into EAU mice. Additionally, vascular dilation and infiltration of CD3~＋CD4~＋T cells were found in the eyes and livers of EAU mice. In conclusion, our findings suggest that liver injury could occur in EAU. This liver injury may be associated with increased CD3~＋CD4~＋T cells, which may infiltrate into liver through circulatory system.