摘要
人尿源性干细胞(human urine-derived stem cells,hUSCs)是从人尿液中通过常温离心分离培养出来的具有良好增殖活性和多向分化能力的成体干细胞,具有间充质干细胞的生物学特性,其在组织器官修复、疾病治疗、药物活性及毒性替代筛选等领域均有重要的应用前景,且已能实现多种途径向尿源性多潜能干细胞(urine-induced pluripotent stem cells,u-iPSCs)转化,但在研究过程中发现仍然存在一些值得深入研究的问题,如人尿液源性干细胞的来源尚不明确,定向诱导多潜能干细胞分化的条件选择及如何提高重编程效率等.本文对hUSCs的来源、分离培养方法、生物学特性及其应用研究最新进展进行综述,总结了由hUSCs向u-iPSCc诱导的方法及其应用前景,为hUSCs的研究和应用提供参考.
Human urine-derived stem cells(hUSCs) are somatic stem cells isolated from fresh urine by the way of centrifugation combined with adherence screening, which are with strong proliferation and multi-differentiation capacity, and have all kinds of similar biological characters as mesenchymal stem cells. HUSCs have the potential of playing an important role in reconstitution of damaged organs and tissues, treatment of the diseases and drug activity and toxicity screening, and urine-induced pluripotent stem cells(u-iPSCs) have been induced by many ways from hUSCs. However, several questions need to be answered prior to developing a technology for drug screen with hUSCs and u-iPSCs, such as the cell source of hUSCs, directional differentiation and efficiency of iPSCs reprogramming. In this review, recent progress in cell source, cell isolation and cultivation, cell biological characteristics, and the application of hUSCs were studied, especially various ways of inducing hUSCs to u-iPSCs and the possible application prospect were summarize, providing a reference for further study and application of hUSCs in drug development and toxicity testing.
作者
周明
孙震晓
ZHOU Ming;SUN Zhen-Xiao(Life Science College, Beijing University of Chinese Medicine, Beijing 100029, China)
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2017年第12期1103-1109,共7页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金资助项目(81473418)
国家林业局野生动植物保护项目(2012-2016)
北京中医药大学东直门医院"111"协同创新院际合作项目(2016-DZM111-ZY008)~~
关键词
人尿源性干细胞
分离培养
生物学特征
应用
尿源性多潜能干细胞
human urine-derived stem cells, cell culture, biological characteristics, application, urine-inducedpluripotent stem cells