三七总甙及甲泼尼龙对实验大鼠肺纤维化的干预作用及机制的初步探讨

The mechanisms and effects of panax notoginside and methylprednisolone in a rat model of pulmonary fibrosis

目的 观察三七总甙 (PNS)及甲泼尼龙对实验大鼠肺纤维化的抑制作用。方法 大鼠随机分为肺纤维化模型组、PNS治疗组和甲泼尼龙治疗组。气管内注入博莱霉素造成动物模型后 ,于当天开始每天给药 ,分别于 1,3,7,14 ,2 8d处死 ,取肺组织和血浆 ,行嗜伊红染色 ,免疫组化测定肺组织Ⅰ、Ⅲ型胶原含量及血浆巨噬细胞炎症蛋白 1α(MIP 1α)、单核细胞趋化蛋白 1(MCP 1)含量。结果与模型组比较两治疗组肺纤维化程度明显减轻 ;肺组织Ⅰ、Ⅲ型胶原含量显著低于模型组 (P <0 0 5 ) ;MIP 1α和MCP 1含量于模型组 3d时升高 ,以后逐渐下降 ,接近正常水平。结论 PNS和甲泼尼龙能有效抑制博莱霉素引起的肺纤维化形成。

Objective To study the effects of panax notoginside (PNS) and methylprednisolone (solu medrol) on the development of pulmonary fibrosis and the possible underlining mechanisms Methods Rats were divided into 3 groups: pulmonary fibrosis group, PNS treated group and solu medrol treated group. On days 1, 3, 7, 14, and 28 after bleomycin treatment, 5 rats in each group were killed and the lungs and plasma were harvested for histopathological studies, immunohistochemical determination of collagen Ⅰ and Ⅲ and ELISA detection of MIP 1α and MCP 1 Results The degrees of fibrosis , the levels of collagen Ⅰ and Ⅲ, and the levels of MIP 1α and MCP 1 decreased significantly in the PNS and solu medrol treated groups as compared with the fibrosis group ( P <0.05) Conclusion Both PNS and solu medrol are effective in modulating the process of pulmonary fibrosis induced by bleomycin in rats.