化学致癌物-MNNG对培养牛胰管上皮作用的研究

EFFECTS OF N-METHYL-N-NITROSO-N-NITROGUANIDINE (MNNG) ON ORGAN-CULTURED BOVINE PANCREATIC DUCTS

以化学致癌物-MNNG对长期培养的牛胰管上皮的作用进行观察。光镜和透射电镜下观察结果,牛胰管上皮出现粘液细胞、复层、结节状、乳头样和异型增生,上皮下腺管不规则增生。增生细胞以核和核仁的异型性变化为特点,并观察到早期癌样病变。

Long-term organ culture of bovine pancreatic ducts and the carcinogenesisof MNNG were reported. Under light and transmission electron microscope,epithelial hyperplasia with presence of sqamous, nodular and papillary structureand aplastic hyperplasia were found in MNNC-treated group.Sheets of atypicalcells forming small irregular lumens and diluting glands were also present.Cytologically and ultrostructrually, the proliferated cells had many dysplasticfeatures, characterized by dysplasia of nuclei and nucleoli. Besides, early carcinoma-like lesion was seen. Experiments showed that organ culture of bovine pancreaticduct can serve as an experimental model for investigation of etiology of pan-creatic carcinoma and mechanism of carcinogenesis in vitro.