利用胶质细胞滋养层进行分离式原代神经元培养

Method of separate culture of primary neurons using glial feeder layer

为使原代培养神经元能长期健康地生长发育 ,并且易于分离、纯化 ,本文利用胶质细胞滋养层进行胶质细胞与神经元分离式培养。结果表明 ,此方法可靠易行 ,所培养的神经元具有纯度高、健康及存活期长的优点 。

In order to obtain primarily cultured neurons that can live longer and are healthy, and easy to be isolated and purified, the glial feeder layers are used for culturing the primary neurons separated from the glial cells. The result shows that this method is reliable and easy to be handled. The cultured neurons are pure, and healthy. They can survive longer and have potential to be utilized in many relevant studies.