橙皮素A的NMR研究

NMR STUDY OF CITRUSIN A

橙皮素A是从中药北沙参 (GlehnialittoralisFr.SchmidtexMiquel)的正丁醇萃取物中分离得到的一种 8 O 4′型异木脂素苷类化合物 .通过化学方法和波谱分析鉴定了该化合物的结构 .采用 2DNMR技术对其NMR信号进行了全归属 .DMSO d6 代替CD3OD作为溶剂 ,并利用重水交换 ,能准确读出偶合常数J7。

A 8 O 4′ neolignan glycoside, citrusin A, was isolated from the BuOH soluble fraction of the ethanol extract of Glehnia littoralis . In addition to conventional 1D NMR methods, 2D NMR techniques (COSY, HMQC and HMBC) were used for the proton and carbon signals assignments. The difficulty in determining J 7,8 values could be overcomed by using DMSO d\-6 and D 2O instead of CD 3OD as a solvent.