大黄蛰虫丸对小鼠酒精性肝纤维化损伤的保护作用

Protective effects of Dahuang Zhechong Pills on mice with alcohol-induced liver fibrosis

目的探讨大黄蛰虫丸(熟大黄、土鳖虫、水蛭,等)对小鼠酒精性肝纤维化损伤的保护作用及机制。方法以C57BL/6雄性小鼠为研究对象,构建酒精性纤维化损伤模型,用大黄蛰虫丸进行干预。检测血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、白介素6(IL-6)、白介素10(IL-10)、干扰素γ(IFN-γ)、肿瘤坏死因子-α(TNF-α)水平,取肝组织做I型胶原蛋白(COL-1)荧光染色、TUNEL染色和切割半胱天冬酶-3(CC3)表达的测定。结果与模型组比较,大黄蛰虫丸组小鼠血清中ALT、AST、IL-6、IFN-γ、TNF-α水平均下降,IL-10水平升高。肝脏COL-1水平下降,肝细胞凋亡减少和CC3表达降低。结论大黄蛰虫丸通过调节炎症因子的水平,减少COL-1的沉淀,抑制肝细胞的凋亡,从而对小鼠酒精性肝纤维化损伤产生保护作用。

AIM To investigate the protective effects of Dahuang Zhechong（DHZC） Pills（Rhei Radix et Rhizoma,Eupolyphaga seu Steleophaga,Hirudo,etc.） against alcoholic liver fibrosis（ALF） injury in mice and to explore the underlying mechanisms. METHODS C57 BL/6 male mice were used to build up ALF injury model,intervened with DHZC Pills. The serum of mice was examined for changes in alanine transaminase（ALT）,aspartate aminotransferase（AST）,interleukin-6（IL-6）,interleukin-10（IL-10）,interferon-γ（IFN-γ） and tumor necrosis factor-α（TNF-α）. Simultaneously,the deposit of collagen 1（COL-1） and apoptotic cell death in liver tissues were analyzed by immunofluorescent and TUNEL assay,respectively. The expressions of cleaved caspase-3（CC3） in livers were measured by Western blot. RESULTS Compared with the model group,the levels of serum ALT,AST,IL-6,IFN-γ and TNF-α of mice in DHZC group were decreased significantly. And the level of serum IL-10 of mice in DHZC group was increased significantly. Mice in DHZC group had higher rates of COL-1 deposition and apoptotic cell death in liver tissues than those in the model group. Mice treated with DHZC Pills showed lower expression of CC3. CONCLUSION DHZC Pills confers protection against ALF injury in mice by inhibiting the generation of COL-1 and down-regulating apoptosis of liver cells death as a result of adjusting the levels of inflammatory factors.