水马桑质量标准的研究

Quality standard for Weigela japonica var. sinica

目的建立水马桑Weigela japonica Thunb.var.sinica(Rehd.)Bailey的质量标准。方法对该药材进行性状、显微鉴定后,采用TLC法作定性鉴别;根据《中国药典》方法,检测水分、总灰分、酸不溶性灰分、浸出物含有量;通过HPLC、UV法,分别测定东莨菪内酯、总香豆素含有量。结果水马桑性状和显微特征可与同属其他植物区分开。TLC斑点清晰,分离度良好。水分、总灰分、酸不溶性灰分、水溶性浸出物、醇溶性浸出物含有量分别为不超过12.0%、不超过2.0%、不超过0.5%、不低于5.0%、不低于4.5%。东莨菪内酯、总香豆素分别在1.25~40.0μg/m L(r=0.999 7)、2.0~64.0μg/m L(r=0.999 9)范围内线性关系良好,平均加样回收率分别为98.19%(RSD=0.90%)、99.21%(RSD=2.5%)。结论该方法准确可靠,可用于水马桑的质量控制。

AIM To establish the quality standard for Weigela japonica Thunb. var. sinica（Rehd.） Bailey（W. j.）. METHODS TLC was adopted in this medicinal material＇s qualitative identification after morphological identification and microscopic identification. The contents of water,total ash,acid-insoluble ash and extracts were detected according to Chinese Pharmacopoeia methods. Then the contents of scopoletin and total coumarins were determined by HPLC and UV,respectively. RESULTS The morphologies and microscopic characters of W. j.could be distinguished from other same generic plants. The clear TLC spot displayed good resolution. The contents of water,total ash,acid-insoluble ash,water-soluble extract and acid-soluble extract were no more than 12. 0%,no more than 2. 0%,no more than 0. 5%,no less than 5. 0% and no less than 4. 5%,respectively. Scopoletin and total coumarins showed good linear relationships within the ranges of 1. 25-40. 0 μg/m L（r = 0. 999 7） and2. 0-64. 0 μg/m L（r = 0. 999 9）,whose average recoveries were 98. 19%（RSD = 0. 90%） and 99. 21%（RSD = 2. 5%）,respectively. CONCLUSION This accurate and reliable method can be used for the quality control of W. j..