NIRS结合PLS算法快速测定西洋参饮片中人参皂苷Rg_1、Re、Rb_1的总含量

Rapid Determination of Ginsenoside Rg_1,Re,Rb_1 in Panax quinquefolius Pieces by NIRS Combined with PLS Algorithm

目的:建立快速测定西洋参饮片中人参皂苷Rg_1、Re、Rb_1总含量的方法。方法:采用高效液相色谱法测定饮片中人参皂苷Rg_1、Re、Rb_1的总含量(作为参考值)。采用红外漫反射光谱技术(NIRS)结合偏最小二乘法(PLS)建立饮片中人参皂苷Rg_1、Re、Rb_1总定量模型:根据参考值采集62份饮片样品,以标准归一化法联合一阶导数法预处理光谱,饮片样品中人参皂苷Rg_1、Re、Rb_1总含量测定最佳波段为7 664.23~5 236.05 cm^(-1)。结果:饮片样品中人参皂苷Rg_1、Re、Rb_1总含量测定方法学验证符合要求。人参皂苷Rg_1、Re、Rb_1总定量模型的校正集相关系数为0.991 03,校正均方差为0.010 26。结论:该方法快速准确、简便无污染,可用于西洋参饮片中人参皂苷Rg_1、Re、Rb_1总含量的快速测定。

OBJECTIVE： To establish the method for rapid determination of ginsenoside Rg1, Re, Rb1 in Panax quinquefolius crude slices. METHODS： HPLC method was adopted to determine the total contents of ginsenoside Rg1, Re, Rb1 （as reference value）. NIRS combined PLS algorithm were adopted to establish total quantitative correction model of ginsenoside Rg1, Re, Rb1. According to the reference, 62 samples were collected. The spectrum was pretreated with multivariate scattering correction method combined with first order derivative method. The optimal ranges of wave band for ginsenoside Rg1, Re, Rb1 were 7 664.23-5 236.05 cm-1. RESULTS： Methodology validation for total content determination of ginsenoside Rg1, Re, Rb1 was in line with the requirements. For total quantitative correction model of ginsenoside Rg1, Re, Rb1, related correction set coefficient was 0.991 03, corrected mean square deviation 0.010 26. CONCLUSIONS： The method is rapid, accurate, simple and free of contamination. It can be used for rapid determination of ginsenoside Rg1, Re, Rb1 in P. quinquefolius crude slices.