接枝RGD多肽磷灰石-硅灰石材料的矿化和生物学特性

Mineralization and biological properties of apatite-wollastonite grafting RGD peptide

应用等离子辅助化学接枝方法在磷灰石-硅灰石(AW)生物活性玻璃的表面接枝精氨酸-甘氨酸-天冬氨酸(RGD)多肽。采用模拟体液(SBF)浸泡方法研究了AW表面接枝RGD基团对材料体外矿化特性的影响。SEM和EDS检测结果表明,RGD多肽的引入有利于羟基磷灰石(HA)的沉积,能够增强RGD-AW复合材料的体外矿化能力,HA形貌为蠕虫状。材料MG-63细胞共培养实验以及材料新西兰成年大白兔体内植入实验的结果表明,表面化学接枝RGD多肽的RGD-AW复合材料能够显著地促进类成骨细胞的黏附和铺展,并且在2周、4周和8周时均能够加速新骨的生成及骨组织结构和功能的重建。

Arginine-glycine-aspartic（RGD）peptide was grafted to the surface of apatite-wollastonite（AW）via plasma-aided treatment.The bioactivity of the RGD-AW was investigated by SEM and EDS after immersing in simulated body fluid（SBF）.The results show that modifying AW with RGD peptide enhance the HA deposition and in vitro mineralization properties,and the HA shows warm-like appearance.In order to survey the in vitro and in vivo biocompatibility of RGD-AW,MG-63 osteoblast-like cells were employed to co-culture with these two materials and animal test was carried out to evaluate the bone regenerative properties of RGD-AW materials.The fluorescence microscope result shows that RGD-AW improves the cell adhesion and spreading compared with AW.The histological evaluation of RGD-AW shows that the bone regeneration and remodeling process are significantly enhanced compared with the original AW after 2 weeks,4 weeks and 8 weeks implantation,respectively.And the hematopoietic function reconstruction which is along with the structure remodeling is only observed on RGD-AW composite after 8 weeks implantation.