负载BMP-2和BSA的丝素蛋白/聚左旋乳酸-己内酯三维纤维屏障膜的制备及性能

Preparation and characterization of 3D fibrous barrier mat of silk fibroin/poly(L-lactide-co-caprolactone)loaded with BMP-2 and BSA

利用骨形态发生蛋白-2(BMP-2)的骨诱导作用和牛血清白蛋白(BSA)的稳定作用,结合同轴静电纺丝技术和共混静电纺丝技术,制备了三组纤维膜,包括同时携载BMP-2和BSA的丝素蛋白(SF)/聚左旋乳酸-己内酯(PLCL)同轴载药纤维膜(BMP-2-BSA@SF/PLCL,Mat A);同时携载BMP-2和BSA的SF/PLCL共混载药纤维膜(BMP-2-BSA-SF/PLCL,Mat B)和非载药SF/PLCL纤维膜(SF/PLCL,Mat C)。通过研究纤维膜的表观形态,得出三种纤维膜均呈均匀网状结构,其中Mat A组具有稳定的核层结构;理化性能研究证实三组纤维的接触角和力学性能依次增大;体外细胞实验和膜屏障实验表明,三组纤维膜均具有良好的生物相容性和屏障功能,且具有药物缓释作用的组纤Mat A维膜能够更加有效地刺激细胞生长和早期骨向分化。所制备的BMP-2-BSA@SF/PLCL能够满足引导性骨组织再生(GBR)生物膜的基本要求,是促进骨缺损修复的理想载体。

Due to theosteoinduction of the bone morphogenetic protein-2（BMP-2）and stabilization of bovine serum albumin（BSA）,three types of new biological mat was fabricated,namely silk fibroin（SF）/poly（L-lactideco-caprolactone）（PLCL）mat loaded with BMP-2 and BSA by coaxial electrospinning（BMP-2-BSA@SF/PLCL,Mat A）,SF/PLCL mat loaded with BMP-2 and BSA by blend electrospinning（BMP-2-BSA-SF/PLCL,Mat B）,non-loaded SF/PLCL mat（SF/PLCL,Mat C）.The results of apparent characteristics show all the fibrous mats present homogeneous network structure,and the core-shell structure is formed only in Mat A.The physicochemical properties results show contact angle and mechanical properties of Mat A,Mat B and Mat C increase in turn.Although each group has good biocompatibility and barrier function,Mat A which has the ability of sustained drugrelease can stimulate growth and osteogenic differentiation of cells more effectively.The BMP-2-BSA@SF/PLCL fibrous mat can meet the requirements of guided bone regeneration（GBR）and will be used as the ideal carrier for bone defect repair.