广叶绣球菌超微粉抗肿瘤作用研究

A study on antitumor effects of ultramicro powder of Sparassis latifolia

广叶绣球菌(荷仙菇-H)是近年来新培养的一种食药用真菌,属担子菌门、多孔菌目、绣球菌科、绣球菌属。本文对其超微粉的抗肿瘤作用及机制进行了研究。建立了H22荷瘤小鼠肿瘤移植模型,设置空白对照组、模型组、环磷酰胺阳性组、广叶绣球菌超微粉高、中、低3个剂量组(1 000,500和100mg/kg),连续给予相应药物10d,计算H22荷瘤小鼠的抑瘤率、体质量、胸腺指数、脾脏指数以及肝脏指数,对各组小鼠的瘤块进行组织病理学研究,并对血清中的SOD和IFN-γ含量进行了测定。结果发现广叶绣球菌超微粉3个给药组的抑瘤率均大于40%,高剂量组抑瘤率最高为54%;可增加小鼠体质量;提高胸腺指数、脾脏指数和肝脏指数;给药组SOD值均高于模型组,说明广叶绣球菌超微粉可以起到抗氧化的作用;给药组IFN-γ值均高于模型组,给药组VEGF值均低于模型组,说明广叶绣球菌超微粉能够提高机体免疫能力。因此,广叶绣球菌超微粉的抗肿瘤作用明显,可能是通过改善机体免疫功能和抗氧化能力,抑制了肿瘤细胞,达到抗肿瘤的功效。

Sparossis Iotifolia is a commercially new edible and medicinal mushroom. The antitumor effects of ultramicro powder of Sparassis latifolia （UPSL） are studied. H22 tumor bearing mice models were established. The experimental treatment groupsncluding control group, model group, cytoxan positive control group, high-dose group （1000mg/kg）, mid-dose group （500mg/kg） and low-dose group （lOOmg/kg） of UPSL were set up. The mice were orally treated with UPSL daily for consecutive 20 days. The increase of the tumor inhibitory rate, tumor weight, body weight, thymus, spleen and liver indexes were calculated The histopathological study of the H22 tumor bearing mice was carried out. The content of SOD and IFN-V in blood serum were detected. The tumor inhibitory rate of the three experimental groups treated with UPSL increased by more than 40%, and the highest was 54% in the high-dose group. UPSL increased the body weight, and thymus, spleen and liver indexes of the mice. The SOD value of the experimental groups treated with UPSL was higher than that of the model group, indicating that UPSL had an mportant role in antioxidation. The IFN-γ value of the experimental groups treated with UPSL was higher than that of the mode group, and the VEGF value of the experimental groups treated with UPSL was lower than that of the model group, indicating that UPSL improved immunity of the mice. Our experiments draw the conclusion that UPSL seems effective in improving the immune function and antioxidant capacities and inhibiting tumor cell of the mice.