分离自北京水系的疫霉属三个中国新记录种

Three Chinese new records of Phytophthora isolated from Beijing water system

疫霉是一类世界性分布的卵菌,目前广泛承认有120种,其中很多存在于湖泊、溪流等水系中。本研究通过叶饵法从北京水系中诱集疫霉,共分离到疫霉菌株463株,利用Single-strand conformation polymorphism(SSCP)进行分析,筛选出不同基因型的代表菌株。依据形态学特征及β-tubulin基因序列和ITS序列对这些菌株进行种类鉴定,发现3种国内新记录种,分别为节水疫霉Phytophthora gonapodyides、北方疫霉Phytophthora borealis和波斯疫霉Phytophthora parsiana。

Four hundred and sixty-three isolates of Phytophthora were obtained from Beijing water system by using leaf bating method. Differ Based on mor parsiana were ent genotypes were screened out us phological characters and the sequ identified as new records in China. ng PCR-based single-strand conformation polymorphism （PCR-SSCP） analysis ence of β-tubulin gene and rDNA-ITS, P. gonapodyides, P. borealis and P.