浅谈凝血功能测定的影响因素

Influential Factors of Blood Coagulation Function Determination

目的:探讨影响凝血功能检测的检测前采集因素、检测中标本状态和操作因素、治疗中药物影响因素。方法:选择某院住院病人及门诊健康体检病人的标本检测凝血功能,对标本不合格者重新抽血或排查影响因素重新检测。结果:(1)标本量不足2ml者PT结果(14.3±2.0)s、APTT结果(45.2±3.9)s、TT结果(26.3±3.2)s显著高于重抽血后合格标本测定结果,组间差异具有统计学意义(P<0.05);(2)离心5min测定PT结果(10.2±1.67)s、APTT结果(24.5±1.30)s显著低于离心10min测定结果,组间差异具有统计学意义(P<0.05)。溶血标本PT结果(9.2±1.1)s、APTT结果(26.1±3.5)s、TT结果(23.1±2.2)s与重抽血后的合格标本测定结果,组间差异具有统计学意义(P<0.05)。有凝块标本与正常标本有明显统计学差异(P<0.05)。结论:标本的采集、离心、标本状态不合格、标本处理不当以及药物治疗都对凝血功能测定有很大影响。

Objective： To investigate the factors influencing the detection of coagulation function before the test, the sample status and operation factors in the test, and the factors influencing the drug treatment. Methods： The blood coagulation function of patients and outpatient physical examination patients was detected,and if the specimen was not eligible, the specimen must draw new blood and and the influencing factor was tested.Resuits：（1）Resuhs of PTT（14.3±2.0） s, APTT （45.2±3.9） s and TT results （26.3±3.2） s in specimens with less than 2 ml were significantly higher than those of qualified specimens after re drawing blood test,the difference between groups was statistically significant （P〈0.05） ;（2）The results of PT （10.2 ±1.67） s and APTT results （24.5 ± 1.30） s determined by centrifugation for 5 minutes were significantly lower than those measured by centrifugation for 10 minutes （P〈0.05）.There was statistical significance between the results of haemolysis samples PT （9.2±1.1） s, APTT （26.1±3.5） s, TT （23.1±2.2） s and the qualified samples after re drawing blood test （P〈0.05）.There was significant difference between clot speci- men and normal specimen （P 〈0.05）.Conclusion ： The specimen collection, centrifugal, unqualified specimen condition, improper handling specimens and drug treatment have a great influence on the determination of blood coagulation function.