基于UHPLC-TOF-MS技术建立金线莲的指纹图谱及金线莲苷的含量测定方法

Establishment of Fingerprint and Determination of Kinsenoside of Herba Anoectochili from Different Sources by UHPLC-TOF-MS

目的：建立野生、组织培养和种植等14批不同来源金线莲药材的超高效液相色谱-飞行时间质谱（UHPLC-TOFMS）指纹图谱,建立金线莲苷的UHPLC-TOF-MS含量测定方法,为完善金线莲药材的质量标准提供实验依据。方法：依据高分辨质谱提供的精确相对分子质量鉴定化学成分,选择[M＋HCOO]-作为专属离子对金线莲苷进行含量测定。采用SPSS22.0软件对各批次药材中化学成分指纹图谱进行相似度考察和聚类分析,评价不同因素对金线莲药材质量的影响。结果：建立了金线莲药材UHPLC-TOF-MS指纹图谱并从中指认出36个化学成分,包括金线莲苷类5个、黄酮类18个、核苷类5个、有机酸类6个、长寿花糖苷C和天麻苷各1个。指纹图谱相似度考察、聚类分析及金线莲苷含量测定结果表明对药材中化学成分种类及含量产生显著影响的因素为产地和栽培时间。金线莲苷质量分数处于4.85%-16.18%。结论：建立的金线莲化学成分指纹图谱及金线莲苷含量测定方法稳定可行,为金线莲药材的质量评价提供了切实可行的检测方法。

Objective： To provide chemical evidence for the quality control of Herba Anoectochili through chemical profiling and quantitative analysis of 14 batches of herbs by UHPLC-TOF-MS technique. Method：Chemical constituents were profiled by UHPLC-TOF-MS fingerprint. Compounds in the fingerprint were assigned on the basis of accurate mass. The contents of kinsenoside was determined by using [M ＋ HCOO]-ion. The obtained fingerprints of 14 batches of Herba Anoectochili were evaluated by similarity investigation and cluster analysis. Result： The UHPLC-TOF-MS fingerprint of Herba Anoectochili was established and thirty-six chemical constituents in the fingerprint were identified. The chemical profiling and quantitative analysis showed that the chemical components of Herba Anoectochili were significantly influenced by place of origin and cultivation time.The content range of kinsenoside was 4. 85%-16. 18%. Conclusion： The established fingerprint and determination of kinsenoside is feasible and stable,which is suitable for the quality control of Herba Anoectochili.