气滞血瘀证动物模型研究现状

Current Status of Animal Model Research of Qi Stagnation and Blood Stasis Syndrome

气滞血瘀证是临床常见证型,它的形成与饮食、情绪、环境等因素有关。随着现代人们生活节奏的加快和生活压力的加大,情志不舒在所难免,再加上饮食油腻等方面的原因,很容易引发气滞血瘀型疾病。临床研究和治疗大都停留在外部宏观手段和技术上,很难对证型发展的微观改变及证型演变规律进行深入系统的了解,这就需要制作和临床发病机制相关的动物模型。为了了解近些年气滞血瘀证及病证结合动物模型研究的现状,本文就造模方法、检测指标及造模原理等方面进行综述。气滞血瘀证常见的造模方法有肾上腺素法、右旋糖酐法、声光电复合刺激法、束缚法、夹尾法、高脂寒凝法、睡眠剥夺法等。模型成功与否的验证指标主要为血液流变学指标,其次还有旷场试验、微循环、体重、神经递质、血管内皮因子等方面的检测。常见的气滞血瘀证病证结合模型有子宫内膜异位症气滞血瘀证模型、气滞血瘀证妇科疾病特点模型、糖尿病气滞血瘀证模型、非小细胞肺癌气滞血瘀证模型、气滞血瘀型慢性非细菌性前列腺炎等。不同的造模方法应用不同,本文通过将近些年造模方式、原理、检测指标进行汇总,为规范气滞血瘀证动物模型提供基础。

Syndrome of blood stasis with Qi stagnation is a common clinical syndrome and the formation is closely related to the diet,mood,environment and other factors. With the quickening pace of modern life and the increasing pressures of life,emotional discomfort is inevitable. In addition,greasy diet and other factors are easy to cause Qi stagnation and blood stasis diseases. Clinical research and treatment most stay in the external macro-means and technology,so it is difficult to understand systematically and deeply about the microcosmic changes of syndrome development and the rule of syndrome evolution. This needs to establish animal models that are related to the clinical pathogenesis. In order to understand the research status about animal models of Qi stagnation and blood stasis syndrome and disease-syndrome combination,the modeling methods,model validation and principles were reviewed in this paper. The common modeling methods of Qi stagnation and blood stasis syndrome include adrenaline method,dextran method,acousto-optic complex stimulation,binding method,clipping tail method,high fat plus cold coagulation,sleep deprivation and so on. The hemorheology indexes are the leading indicator of model validation,followed by open field test,and microcirculation,weight,neurotransmitter,vascular endothelial factor and other tests. The common disease-syndrome combination models of Qi stagnation and blood stasis syndrome include the models of Qi stagnation and blood stasis syndrome with endometriosis,Qi stagnation and blood stasis syndrome with gynecological disease characteristics,Qi stagnation and blood stasis syndrome with diabetes,Qi stagnation and blood stasis syndrome with non-small cell lung cancer,and Qi stagnation and blood stasis syndrome with chronic non-bacterial prostatitis. Different modeling methods are used in the different applications.We summarized the modeling methods,principles and testing indicators in recent years to standardize the animal model of Qi stagnation blood stasis syndrome.