酶免分析仪孵育过程的分段温控方法研究

Research on segmentation temperature control method of incubation process for ELISA analyzer

针对酶免分析仪孵育过程耗时过长、温度稳定性差导致试剂反应差异性较大的问题,提出了一种基于改进型数字PID的分段温度控制方法。首先对孵育过程中试剂温度及孵育位温度变化曲线特性进行分析,确定预升温段及恒温段的分段控制温度参数;然后根据温度参数及各段控制指标,分别对孵育过程的各温度变化段采用不同算法分阶段进行控制;最后基于酶免分析仪框架搭建了温控实验系统,对反应液温度上升时间及温度稳定性进行验证。实验结果表明,试剂由21℃升至37.5℃的温度上升时间为239±5 s,恒温阶段的温度偏差小于±0.2℃。该方法可实现反应液迅速升温并且稳定恒温,保证了孵育过程中生物酶反应的快速及稳定。

The testing efficiency of ELISA analyzer is seriously affected the time-consuming incubation and the accuracy will be reduced by the differences in the degree of reagent reaction due to temperature instability. Aiming at these problems, a method of segmentation incubation based on the improved digital PID algorithm was proposed. Firstly, the temperature characteristics of reagent and incubation system were analyzed and the parameters in pre-heating and constant temperature stage were determined. Secondly, different algorithms were applied in the different sections of incubation process according to the parameters and control indicators. And then, the experiment system was set based on ELISA analyzer framework to verify the time of temperature rising and stability of reagent. The experiment results indicate that the reagent is heated up from 21 ℃to 37.5 ℃1 in 239 ± 5 seconds, meanwhile, the temperature deviation of isothermal segment is less than ± 0.2 ℃. The method proposed in this paper achieves fast and stable temperature control and ensures enzyme reaction fast and stable.