继发性胆脂瘤中CCL27的定位及检测

Expression of chemokine ligand 27 in secondary middle ear cholesteatoma

目的明确趋化因子CCL27在继发性中耳胆脂瘤中的病理学表达,探讨可能的临床意义。方法应用免疫组织化学SP法检测CCL27在继发性中耳胆脂瘤(n=30)及正常耳后皮肤组织(n=30)的表达情况,并分析该趋化因子与胆脂瘤病理学之间的联系。结果继发性胆脂瘤、耳后皮肤组织中CCL27的阳性率分别为6.7%和33.3%,差异有统计学意义(P=0.021)。结论继发性胆脂瘤组织中CCL27的低表达可能与胆脂瘤的增殖过于活跃有关,CCL27可能在继发性中耳胆脂瘤发病过程中扮演重要角色。

Objective To explore the pathological expression of chemokine ligand 27 （ CCL27） and discuss its possible clinical significance in secondary middle ear cholesteatoma. Methods The SP immunohistochemical staining technique was applied to detect the expression of CCL27 in 30 samples of secondary middle ear cholesteatoma tissue and 30 sam-ples of normal auricle skin. Results Of the secondary middle ear cholesteatoma tissue samples, 6.7% were positive for CCL27 expression, whereas 33.3% of the normal auricle skin samples were positive for CCL27 expression. CCL27 was expressed less commonly in the former, and there was a significant difference in the rate of expression between the two groups （ P=0.021） . Conclusion The lower expression of CCL27 may be correlated with the hyperproliferative nature of the secondary middle ear cholesteatoma epithelium. CCL27 may play a significant role in the development of seconda-ry middle ear cholesteatoma.