脑胶质瘤组织中P-gp、MRP、BCRP的表达及临床意义

Expression and clinical significance of p-gp,MRP and BCRP in glioma tissues

目的探讨脑胶质瘤组织中P-糖蛋白(P-gp)、多药抗药性相关蛋白(MRP)、乳腺癌耐药相关蛋白(BCRP)的表达及临床意义。方法选择2007年7月~2016年7月于武安市第一人民医院手术切除的脑胶质瘤标本56例作为观察组,同期脑外伤的正常脑组织10例作为对照组。应用免疫组织化学SP法检测两组标本中的P-gp、MRP、BCRP表达情况,并分析其与脑胶质瘤患者的临床病理特征的关系。结果观察组P-gp、MRP、BCRP阳性表达率分别为42.86%(24/56)、76.79%(43/56)、46.43%(26/56),均明显低于对照组的0.00%(0/10)、10.00%(1/10)、0.00%(0/10)(P<0.01)。脑胶质瘤组织P-gp、MRP、BCRP阳性表达率与肿瘤病理分期、组织学分级有关,病理分期越高,P-gp、MRP、BCRP阳性表达率越高;组织学分级越低,P-gp、MRP、BCRP阳性表达率越高,差异均有统计学意义(P<0.01)。Spearman等级相关分析显示,脑胶质瘤组织P-gp阳性表达率与MRP、BCRP阳性表达率呈正相关(Rs=0.612、0.537,P<0.05)。结论脑胶质瘤组织中P-gp、MRP、BCRP均呈高表达,其表达水平与肿瘤病理分期、组织学分级有关,且三者表达呈正相关。

Objective To investigate the expression and clinical significance of P- glycoprotein （P-gp）, multidrug resistance associated protein （MRP） and multidrug resistance associated protein （BCRP） in brain gliomas. Methods Fifty-six cases of brain glioma resected in the First People＇s Hospital of Wuan from July 2007 to July 2016 were selected as the observation group, and 10 normal brain tissues of brain trauma in the same period were selected as control group. The expressions of P-gp, MRP and BCRP in specimens of two groups were detected by immunohistochemistry （SP）, and the relationship between the expression and clinical pathological features of glioma was analyzed. Results The P-gp, MRP, BCRP positive expression rates of the observation group was 42.86% （24/56）, 76.79% （43/56）, 46.43% （26/56）, respectively, was significantly lower than the control group of 0% （0/10）, 10% （1/10）, 0% （0/10） （P ＜ 0.01）. The positive rates of P-gp, MRP and BCRP in glioma was related to the pathological stage and histological grade of glioma, the higher the pathological stage, the higher the positive expression rate of P-gp, MRP and BCRP. The lower the histological grade, the higher the positive expression rates of P-gp, MRP and BCRP, and the differences were statistically significant （P ＜ 0.01）. Spearman rank correlation analysis showed that the positive rate of P-gp expression in glioma tissues was positively correlated with the positive rates of MRP and BCRP （Rs = 0.612, 0.537, P ＜ 0.05）. Conclusion The expressions of P-gp, MRP and BCRP are highly expressed in gliomas, and the expression level is related to the pathological stage and histological grade, and the three expressions are positively correlated.