透明颤菌血红蛋白基因在酶工程中的应用

The Applications of Vitreoscilla Hemoglobin(vgb) in Enzyme Engineering

透明颤菌的血红蛋白(VHb)具有结合氧的特性,可使专性好氧的革兰氏阴性菌透明颤菌在贫氧环境中生长。目前,VHb可以在很多宿主细胞中表达,通过促进氧的输送增强呼吸和能量代谢,以提高许多有益的代谢产物的产量。本文综述了VHb的结构与功能,以及vgb基因在产酶工程菌中的应用及存在的问题,并对vgb基因在酶工程领域的应用前景进行了展望。

The VHb is thought to aid Vitreoscilla＇s obligately aerobic host＇s survival in the microaerobic environments. Expressing VHb in a variety of heterologous hostsresults substantial increasesin production of a variety of useful products. So this paper reviewed on the structure and function of VHb and the application and existing problems in integrate heterologous bacterial hosts with vgb,and we discussed about the application prospect and development space in the enzyme engineering field.