附子品种的抗病性及根表真菌多样性研究

Study on Disease Resistance of Radix Aconiti Lateralis Praeparata Varieties and their Rhizoplane Fungal Diversities

目的:在系统中药思想指导下,研究附子品种的抗病能力及与外界真菌系统多样性的关系。方法:使用高通量技术构建附子品种的根表真菌文库,分析其多样性指数与附子抗病品质形成的关系。结果:小花叶与瓜叶子型附子之间,以及健康与病害附子之间,其根表土壤的优势真菌种类、群落多样性均存在差异,附子根系影响了土壤真菌的结构组成,阿太菌、总状毛霉等是附子根腐病的致病菌群,而被孢霉属、青霉菌属等又组成病害菌的拮抗菌群。结论:本结果为附子农业的良好品质管理提供理论指导,也丰富了附子品种品质形成的系统学理论与分析方法。

Objective： To study the relationship between the disease resistance of Radix Aconiti Lateralis Praeparata and the diversity of fungi system under the guidance of systematic theory of Chinese herbal medicine. Methods： High-throughput technology was used to build the rhizoplane fungus library of different types of Radix Aconiti Lateralis Praeparata,and then the relationship between the diversity index and the anti-disease capacity was analyzed. Results： There were differences in the dominant strains and the diversity of fungi community for the rhizoplane soils between the Xiao-Hua Radix Aconiti Lateralis Praeparata and the Gua-Ye Radix Aconiti Lateralis Praeparata,the healthy and pathogenic Radix Aconiti Lateralis Praeparata; The root system of Radix Aconiti Lateralis Praeparata affect the structure of soil fungi. Atheli arolfsii,Mucor racemosus were the pathogenic fungi,while the Mortierella,Penicillium are antagonistic fungi to root rot pathogens. Conclusion： The results provide theoretical guidance for good quality management of Radix Aconiti Lateralis Praeparata agriculture,and enrich the theory and analysis methods for the system science of Chinese herbal medicine.