树脂改性玻璃离子Vitrebond的光固化时间对细胞毒性的影响

Effect of curing time on cytotoxicity of Vitrebond

目的:探讨树脂改性玻璃离子Vitrebond不同光固化时间下对细胞毒性的影响,比较琼脂扩散法和MTT法的细胞毒性敏感度。方法:Vitrebond根据说明书制成2mm厚样本,在强度1000mw/cm^2光照下,分成4组,经不同光照时间(0、15、30、45s)后作为实验组,苯酚为阳性对照组,MEM、棉花籽油为阴性对照组。每组各含样本8个,在24和48h后,应用琼脂扩散法,分别评估L929细胞脱色区和L929细胞溶解指数;实验第5天,通过MTT法,比较各组中Vitrebond对L929细胞的细胞毒性。结果:琼脂扩散法中,阳性对照及未光固化组呈现严重细胞毒性,阴性对照及各光固化组无细胞毒性。MTT法中,实验组均有较强细胞毒性,且组间差异无统计学意义(F=1.452,P>0.05)。结论:1000mw/cm^2光照下,Vitrebond的光固化时间对细胞毒性无影响;琼脂扩散法敏感度不如MTT法。

Objective： To investigate the influence of curing time of curing light on the cytotoxicity of Vitrebond, and compare the cytotoxic sensitivity of Agar diffusion test and MTT assay. Methods： Vitrebond of 2 mm thickness were light-cured for 15, 30, 45 seconds with 1 000 mw/cm^2 curing light. The effects of different curing time of Vitrebond on L929 cell were evaluated by Agar diffusion test and MTT assay after 24 h and 48 h. Results： The evaluation of agar diffusion found the positive control and the specimen without any curing induced severe cytotoxity, there was no cytotoxicity in negative control, any of specimens and any of MEM or cottonseed oil extracts. In MTT test, Vitrebond showed a great cytotoxicity whatever the curing time was. There was no sta- tistical difference of cytotoxicity cured for 15, 30, and 45 seconds （F=1.452, P〉0.05）. Conclusion： There are no difference of cytotoxicity of Vitrebond cured with 1 000 mw/cm^2, with different curing time. Agar diffusion test is lower sensitive than MTT test to detect the cytotoxicity.