抗炎通管汤对慢性输卵管炎模型大鼠EGFR和ICAM-1表达的影响

Effect of anti-inflammatory dredging oviduct decoction on EGFR and ICAM-1 levels in rats with chronic salpingitis

目的:探讨抗炎通管汤对慢性输卵管炎大鼠模型输卵管上皮细胞表皮生长因子受体(EGFR)及细胞间黏附分子-1(ICAM-1)表达的影响。方法:采用混合菌株接种法制作慢性输卵管炎大鼠模型。按照随机分组法分为正常组,模型组,金刚藤颗粒组,低、中、高剂量抗炎通管汤组。通过免疫组织化学法和实时荧光定量PCR判断抗炎通管汤如何影响慢性输卵管炎模型大鼠输卵管组织上EGFR、ICAM-1的表达。结果:与正常组比,模型组及各给药组EGFR、EGFR mRNA表达均升高,差异有统计学意义(P<0.05);与模型组比,中、高剂量抗炎通管汤组EGFR、EGFR mRNA表达下降,差异有统计学意义(P<0.05);而金刚藤颗粒组、低剂量抗炎通管汤组EGFR、EGFR mRNA表达差异无统计学意义(P>0.05)。与正常组比,模型组及各给药组ICAM-1、ICAM-1 mRNA表达均升高,差异有统计学意义(P<0.05);与模型组比,中、高剂量抗炎通管汤组ICAM-1、ICAM-1 mRNA表达下降,差异均有统计学意义(P<0.05);金刚藤颗粒组、低剂量抗炎通管汤组ICAM-1、ICAM-1 mRNA表达差异无统计学意义(P>0.05)。结论:抗炎通管汤能有效调控EGFR蛋白在输卵管上皮细胞的过度表达及ICAM-1蛋白的表达,发挥抗炎、疏通输卵管的作用。

Objective： To discuss the effect of anti-inflammatory dredging oviduct decoction on EGFR and ICAM-1 levels in rats with chronic salpingitis. Methods： Using mixed strains inoculation method, rat model with chronic salpingitis was manufactured. After making those models, those rats were divided into normal group, model group, rhizoma smilax china group and anti-inflammatory dredging oviduct decoction groups with different doses. The immunohistochemical and real-time PCR methods was used to observe effect of anti-inflammatory dredging oviduct decoction on EGFR and ICAM-1 levels in rats with chronic salpingi- tis. Results： Compared with normal group, the model group and the others all increasingly expressed EGFR and EGFR mRNA, and there were significant differences between results of two groups （P〈0.05）. Compared with model group, anti-inflammatory dredging oviduct decoction with the medium and high dose lowerd lev- els of EGFR and EGFR mRNA, both significantly decreased （P〈0.05）. Compared with normal group, the model group and the others all increasingly expressed ICAM-1 and ICAM-1 mRNA, and there was significant difference between results of two groups （P〈0.05）. Compared with the model group, anti-inflammatory dredging oviduct decoction with the medium and high dose lowerd levels of ICAM-1 and ICAM-1 mRNA, both significantly decreased （P〈0.05）. Conclusion： Anti-inflammatory dredging oviduct decoction can exert therapeutic effects via inhibiting excessive expression of EGFR and ICAM-1 protein in oviduct epithelial cell.