注意缺陷多动障碍儿童5-HTTLPR长等位基因A-G单核苷酸多态性与静息态脑功能及行为学的相关性

The role of A-G single nucleotide polymorphism in the L allele of the 5-HTTLPR gene of attention deficit hyperactivity disorder: based on resting-state cerebral function

目的:分析注意缺陷多动障碍(ADHD)患儿SLA基因型与SLG基因型间脑低频振荡振幅(ALFF)的差异以及静息态ALFF与行为学的相关性,探讨A-G单核苷酸多态性对5-HTTLPR长等位基因(L)转录活动的影响。方法:22例ADHD患儿通过PCR基因检测技术筛查出SLA基因型(SLA基因组)12例、SLG基因型(SLG基因组)10例,用磁共振扫描仪对患儿进行功能磁共振静息状态扫描,采用ALFF评估静息态脑功能,Conners儿童行为问卷评估行为现状,有意义脑区的ALFF平均信号与Conners儿童行为问卷的各项指标做两两相关分析。结果:SLA基因组在右额上回、左额中回、左脑岛、右边缘扣带回ALFF值高于SLG基因组,其中左额中回的ALFF值与Conners儿童行为问卷的冲动多动评分呈显著负相关(r=-0.359,P=0.020),右边缘扣带回的ALFF值与冲动多动评分、品行问题评分均呈显著负相关(r=-0.479,P=0.024;r=-0.542,P=0.009)。SLA基因组低于SLG基因组的脑区ALFF值差异均无统计学意义。结论:与SLG基因型相比,SLA基因型在大脑的多个区域均表现出更为活跃的功能活动,且倾向于更严重的冲动多动症状和品行问题。

Objective： To study the differences of amplitude of low frequency fluctuation （ALFF） between genotypes of SLA and SLG with attention deficit hyeractivity disorder （ADHD） children using functional mag- netic resonance imaging （fMRI） and the relationship between ALFF and behavioristic. Methods： Screened out the genotypes of SLA and SLG of ADHD children by PCR gene detection technology. And all of the ADHD chil- dren were scanned with fMRI scanner in the resting state, using ALFF to assess the resting state cerebral function. And the Conners children behavior questionnaire was used to assess the behavior status quo. Results： Compared with the SLG genotype, SLA genotype showed ALFF were increased in right superior frontal gyms, left middle frontal gyrus, left insula, right limbic cingulate gyrus, and no brain region with decreased ALFF. The ALFF of left middle frontal gyrus was significantly negative correlative with impulse-hyperactivity ratings of conners children behavior questionnaire, and ALFF of right limbic cingulate gyrus was significantly negative correlative with im- pulse-hyperactivity ratings and conduct problem score. Conclusion： Compared with the SLG genotype, the SLA genotype shows more yauld cerebral functional activities, and tend to be more serious impulse-hyperactivity and conduct problem.