桦褐孔菌对大鼠血管性痴呆的保护作用机制

Study on protective mechanism of Inonotus obliquus against Vascular dementia in rats

目的探讨桦褐孔菌对大鼠血管性痴呆保护作用的潜在机制。方法选用Wistar大鼠,采用反复夹闭大鼠双侧颈总动脉再灌注,合并腹腔注射硝普钠降压法制备VD模型,动物分为正常对照组、模型组、孔菌药物组(0.5、1.0、2.0 g/kg),连续灌胃给药8周,检测大鼠脑组织中自由基及相关酶以及胆碱能系统的变化。结果自由基及相关酶检测结果显示,与模型组相比,桦褐孔菌各剂量组大鼠脑组织中SOD和CAT的活性明显升高,MDA的含量明显降低(P<0.05),GSH-PX的活性及Na+-K+-ATP酶的活性无明显变化,桦褐孔菌大剂量组大鼠脑组织中Ca2+-Mg2+-ATP酶的活性明显升高(P<0.05);胆碱能系统及相关酶检测结果显示,与模型组相比,桦褐孔菌各剂量组大鼠脑组织中乙酰胆碱含量及胆碱酯酶活性均无明显变化。结论桦褐孔菌对VD大鼠保护作用机制可能与增强抗自由基相关酶的活性,增加机体的抗氧化能力,减轻脑组织自由基损伤有关;其作用机制可能与胆碱能系统改善无关。

Objective To observe the protective effect mechanisms of Inonotus obliquus on Vascular dementia（VD） rats. Methods Wistar rats were selected, used repeatedly occlusion of bilateral common carotid arteries and reperfusion, combined with intraperitoneal injection of sodium nitroprusside depressor preparation of VD animal model,randomly divided into control group, model group, IO group（0.5, 1.0, 2.0 g/kg）, and intragastric administration for8 weeks. The changes of free radical and related enzymes and cholinergic system in the brain tissue of rats were detected. Results Free radical and enzyme assay showed that compared with the model group, SOD and CAT activity of brain tissue in rats of Inonotus obliquus groups increased and MDA content decreased（P〈0.05）, Na^＋-K^＋-ATP and the activity of the enzyme GSH-PX activity had no obvious change, inonotusobliquus large dose group（2.0 g/kg）Ca^2＋-Mg^2＋-ATP enzyme activity in rat＇ s brain increased significantly（P〈0.05）. The detection of cholinergic system and related enzymes showed that there were no significant changes in the content of acetylcholine and cholinesterase in the brain tissue of the rats in each dose group compared to the model group. Conclusion The protective mechanism of obliquus obliquus against VD rats may be related to enhancing the activity of anti free radical related enzymes,increasing the antioxidant capacity of the body, and alleviating the damage of free radical in brain tissue; its mechanism maybe irrelevant to choline system.