miRNA-200c在上皮性卵巢癌患者血清中的表达及其与临床特征关系的研究

Expression of miRNA-200c in sera of patients with epithelial ovarian cancer and its relationship with clinical features

目的观察上皮性卵巢癌患者血清中miRNA-200c的表达及其与临床特征的关系。方法应用RTPCR法检测30例上皮性卵巢癌患者(观察组A)、良性上皮性卵巢肿瘤患者(观察组B)、健康志愿者(对照组)血清中miRNA-200c的表达,并比较miRNA-200c在上皮性卵巢癌患者不同FIGO分期、病理类型、分化程度、淋巴结转移状态中的表达。结果观察组A、观察组B及对照组中miRNA-200c表达依次为(2.891±0.183),(1.121±0.126),(0.431±0.069),观察组A miRNA-200c相对表达量明显高于观察组B及对照组,观察组B明显高于对照组,差异均有统计学意义(P<0.05)。在观察组A中,miRNA-200c在卵巢子宫内膜样癌中高表达,随着FIGO分期的进展、分化程度的降低,miRNA-200c的表达呈下调趋势。淋巴结转移阳性的病例中,miRNA-200c表达也明显下调,以上差异均有统计学意义(P<0.05)。结论 miRNA-200c在上皮性卵巢癌的发生、发展的分子机制中可能具有类似致癌基因和抑癌基因的双重性作用。

Objective To observe the expression of miRNA -200c in serum of patients with epithelial ovarian cancer and its relationship with clinical features. Methods Thirty patients with epithelial ovarian cancer （ observation group A）, benign epithelial ovarian tumor as observation group B and healthy volunteers were compared, the expression of miRNA - 200e in different FIGO staging, pathological type, differentiation degree and lymph node metastasis of epitheli- al ovarian cancer were compared. Results The expression of miRNA - 200e in observation group A, observation group B and control group were （2. 891 ±0. 183）, （1. 121 ±0. 126） and （0.431 ±0.069）, respectively. The relative ex- pression of miRNA -200c in observation group A was significantly higher than that in observation group B and control group. Group B was significantly higher than the control group, the difference was statistically significant （ P 〈 0. 05 ）. In observation group A, miRNA -200c was highly expressed in ovarian endometrioid carcinoma, and the expression of miRNA - 200e was down - regulated with the progress of FIGO staging and the degree of differentiation. The expression of mIRNA - 200c was also clown - regulated in lymph node metastasis positive eases, the difference was statistically sig- nificant （ P 〈 0. 05 ）. Conclusion miRNA - 200c may play a dual role in carcinogenesis and tumor suppressor genes of epithelial ovarian carcinogenesis and development.