一次性力竭运动对大鼠PHB1表达及线粒体功能的影响

Effects of exhaustive exercise on the expression of PHB1 and the function of mitochondria in rats

目的:观察一次性力竭运动后大鼠脑、心、骨骼肌组织和线粒体中PHB1含量的变化及对大鼠线粒体功能的影响,探寻PHB1与线粒体功能和能量代谢的关系。方法:健康雄性SD大鼠40只,随机分为2组(n=20):对照组和一次性力竭运动组,大鼠进行一次性急性跑台运动建立力竭运动模型。收集各组大鼠的心、脑和骨骼肌组织样品并提取线粒体,检测其呼吸功能和ROS的变化。用Western blot方法检测组织和线粒体中PHB1蛋白表达水平;用分光光度计检测各器官中ATP含量以及线粒体中复合体V活性(ATP合酶活性)。结果:(1)一次性力竭运动后脑、心肌、骨骼肌中ATP含量显著性降低;(2)一次性力竭运动后脑、心肌、骨骼肌线粒体中复合体V活性、RCR、ROS显著性降低,ST4均显著性升高,ST3无显著性差异。(3)一次性力竭运动后心、脑、骨骼肌线粒体中PHB1的表达显著性减少。(4)通过相关性分析得出:一次性力竭运动后心、脑、骨骼肌中ATP含量与心、脑、骨骼肌中复合体V活性呈正相关;心、脑、骨骼肌中ATP含量和心、脑骨骼肌中PHB1的表达呈正相关。结论:一次性力竭运动后,降低线粒体氧化磷酸化功能,使大鼠脑、骨骼肌线粒体内ROS生成增加,PHB1的表达、ATP含量和复合体V活性均下降。一次性力竭运动使得大鼠线粒体内PHB1表达降低,线粒体功能减弱,机体能量代谢降低。

Objective： To observe the changes of Prohibitinl （PHB1） contents in rat brain, heart, skeletal muscle tissue and mitochondria with acute exhaustive exercise and the effects of acute exhaustive exercise on mitochondrial function in rats, to explore the relationship among PHBI and mitochondrial function and energy metabolism. Methods： Acute exhaustive exercise m^el： The rats carried acute exhaustive exercise after 8 weeks of feeding. The heart, brain and skeletal muscle samples were collected and the mitochondria were collected to detect the changes of respiratory function and reactive oxygen species（ROS）. Tile expression of PHBI protein in tissues and mitochondria was detected by Western blot. The ATP content in the organs and the activity of complexes （ATP synthase activity） in mitochondria were measured by spec- trophotometer. Results： ① The ATP contents of brain, myocardium and skeletal muscle were decreased significantly after acute exhaustive ex- ercise. ②The activities of complex V, respiratory control rates （RCR） and ROS in mitochondria of brain, myocardium and skeletal muscle weir decreased significantly after acute exhaustive exercise, respiration rate state 4（ST4） was increased significantly, at the same time, respi- ration rate state 3 （ST3） had no significant difference. ③ The expression of PHB 1 in mitochondria of skeletal muscle was decreased significantly ＇after acute exhaustive exercise, while there was no significant change in PHBI in myocardial tissue and mitochondria. ④ The correlation anal- ysis showed that the ATP contents in the brain, myocardium and skeletal muscle were positively correlated with the activity of complex V and the expression of PHB1 after acute exhaustive exercise. Conclusion： After acute exhaustive exercise, the mitochondrial oxidative phosphorylation was reduced, the ROS production was increased, the expression of PHB1 was decreased, the ATP content and the activity of complex V were decroased in the brain and skeletal muscle of rats. Acute exhaustive exercise reduced the expression of PHB1 in mitoehondria, decreased mitochondrial function, and reduced energy metabolism.