联合分子标志物检测在预测子宫内膜癌淋巴结转移中的应用

Expression of HER-2,PI3K and CDH4 in Detection of Lymph Node Metastasis in Patients with Endometrial Carcinoma

[目的]探讨HER-2、PI3K、CDH4分子标志物在子宫内膜癌淋巴结转移中的表达及相关性。[方法]选取2013年1月至2014年1月河南省肿瘤医院收治子宫内膜癌患者53例,根据是否发生淋巴结转移分为淋巴结转移组(n=14)和淋巴结未转移组(n=39)。采用免疫组化SP方法检测HER-2、PI3K、CDH4蛋白的表达;采用Logistic回归分析以上检测指标表达量与淋巴结转移之间的关系。[结果]淋巴结转移组HER-2蛋白、PI3K蛋白和CHD4蛋白阳性率均高于淋巴结未转移组(P<0.05)。Logistic回归分析结果显示:PI3K、CDH4蛋白表达量与子宫内膜癌淋巴结转移相关(P<0.05);HER-2蛋白表达量与子宫内膜癌淋巴结转移无明显相关(P>0.05)。[结论]子宫内膜癌患者联合HER-2、PI3K、CDH4蛋白测定能为临床制定个体化治疗方案提供参考依据。

[Objective] To investigate the expression of HER-2,PI3K and CDH4 in endometrial carcinoma patients with lymph node metastasis. [Methods] Fifty three patients with endometrial cancer treated in Henan Cancer Hospital from January 2013 to January 2014 were enrolled in the study,including 14 cases with lymph node metastasis and 39 cases without lymph node metastasis. The expression of HER-2,PI3K and CDH4 protein was detected by immunohistochemistry SP method and the relationship between protein expression and lymph node metastasis was analyzed with Logistic regression. [Results] The positive rate of HER-2,PI3K and CHD4 proteins in lymph metastasis group was higher than that in negative metastasis group（P〈0.05）. Logistic regression analysis showed that the expression of PI3K and CDH4 protein was correlated with lymph node metastasis in endometrial carcinoma（P〈0.05）,but the expression of HER-2 protein had no significant correlation（P〉0.05）. [Conclusion] The expression of PI3K and CDH4 proteins may indicate lymph node metastasis in patients with endometrial cancer .