姜黄素治疗幼年小鼠脑外伤的实验研究

The role of CCM on traumatic brain injury in young mice

目的探讨幼年小鼠脑外伤后应用姜黄素(CCM)治疗是否具有神经保护和促进神经再生的作用。方法将92只C57BL/6小鼠随机分为3组,建立自由落体脑外伤动物模型,并分为假手术(Sham)组,脑外伤+生理盐水(TBI+Vehicle)组,脑外伤+姜黄素(TBI+CCM)组。尼氏染色观察大脑损伤体积,mNSS评分评价小鼠的运动功能,干湿称重法检测小鼠损伤侧大脑水含量,Edu染色观察SGZ区神经再生,Western blot检测β-catenin和GSK3β蛋白表达。结果幼年小鼠脑外伤后,CCM治疗可减少大脑损伤体积,改善运动功能,明显增加Edu阳性细胞数,减少GSK3β的表达,同时增加β-catenin的表达(P<0.05)。结论幼年小鼠脑外伤后应用CCM治疗能间接激活Notch通路,同时具有神经保护和促进神经再生的作用,为今后治疗儿童脑外伤提供新的思路。

Objective To detect whether CCM could protect the brain after TBI. Methods 92 C57BL/6 mice were divided into 3 groups randomly： Sham group,TBI＋Vehicle （NaC1） group and TBI--CCM group. Construct the weight drop model which could mimic TBI. The lesion volume, motor and cognition function, the expression of β-catenin and GSK3β protein, the cell death near injury area and the neurogenesis in SGZ area were detected in each group. Results CCM treatment could decrease the volume of brain injury, improve motor function, significantly increase the number of Edu positive cells, reduce the expression of GSK3β, while increasing the expression of β-catenin （P〈0.05）. Conclusion In the young mice TBI model,CCM decreased the lesion vol ume, promoted the motor and the cognition function, activated Notch pathway indirectivity, reduced the cell death near injury area, increased the neurogenesis in SGZ area and promoted a new method to treat TBI in young mice.