四种Bt蛋白对六种重要鳞翅目害虫的杀虫活性评价

Insecticidal Activity of Four Different Bt Toxins against Six Important Lepidopteran Pests

筛选对靶标害虫高效、低毒的Bt蛋白对未来开发新型Bt生物杀虫剂、转基因抗虫植物新品种等具有重要意义。我们在室内测定了Cry2Ab4、Cry2Ah1、Cry1Ca7及Vip3Aa11四种Bt蛋白对棉铃虫、甜菜夜蛾、斜纹夜蛾、黏虫、玉米螟、二化螟的杀虫效果。结果表明,Cry2Ab4杀虫谱广、对六种鳞翅目的重要害虫都具有很高的杀虫效果,尤其是对棉铃虫、甜菜夜蛾、斜纹夜蛾和玉米螟的毒力效果显著;Cry2Ah1对斜纹夜蛾、甜菜夜蛾具有很好的致死作用,对棉铃虫、玉米螟和二化螟也具有较好的杀虫效果,但对黏虫只有明显的体重抑制作用、致死效果不明显;Cry1Ca7对甜菜夜蛾和斜纹夜蛾有明显的防治效果,其次对棉铃虫、二化螟也有较好的杀虫作用,但对玉米螟毒力效果较差,对黏虫没有毒力作用;Vip3Aa11对甜菜夜蛾和斜纹夜蛾的防治效果好,对玉米螟、棉铃虫和二化螟有较好的防治效果,但对黏虫没有毒力作用。

Selection of Bt toxin with high efficiency against target pests and low harm to non-target creatures is very important for the development of novel Bt bio-insecticide or insecticidal gene in the future. We tested the insecticidal efficiency of 4 different Bt toxins（Cry2 Ab4, Cry2 Ah1, Cry1 Ca7 and Vip3 Aa11） against 6 species of lepidopterans pests, including Helicverpa armigera, Spodoptera exigua, Spodoptera litura, Mythimna separate, Ostrinia furnacalis and Chilo suppressalis. The results showed that Cry2 Ab4 had wide insecticidal spectrum, it had high insecticidal efficiency against all the six species of lepidopteran pests; especially, it had obvious toxicity effect on H. armigera, S. exigua, S. litura and O. furnacalis. Cry2 Ah1 had good lethal effect on H. armigera, S. exigua, S. litura and had certain efficiency against O. furnacalis and C. suppressalis; meanwhile it had significant weight inhibition effect on M. separate rather than lethal effect. Cry1 Ca7 had obvious control efficiency against S. exigua and S. litura, and also good effects on H. armigera and C. suppressalis. Cry1 Ca7 had lower insecticidal efficiency against O. furnacalis and no activity against M. separate. Vip3 Aa11 had good efficiency against S. exigua and S. litura, and adequate effects on O. furnacalis, H. armigera and C. suppressalis, while had no activity against M. separate.