长链非编码RNARP11-307P5.2在喉癌及喉咽癌中的表达特点

Expression of long non-coding RNA--RP11-307P5.2 in laryngeal and hypopharyngeal carcinoma

目的对喉癌及下咽癌中异常表达的lnc RNA进行探讨。方法从Gene Expression Omnibus(GEO)收集喉癌、喉咽癌及头颈部正常黏膜上皮的高通量芯片数据,利用nc FANs筛选出喉癌及喉咽癌中显著异常表达的lnc RNA。收集44例初发喉癌及喉咽癌患者的临床数据及新鲜组织标本,提取RNA,通过RT-PCR验证显著异常表达的lnc RNA。结果共筛查出7个lnc RNA在肿瘤及正常组织的表达量之间存在显著差异,其中仅有RP11-307P5.2在喉癌及喉咽癌组织验证中显著低表达,与电脑筛选结果一致。此外,晚期病例中的RP11-307P5.2表达量较早期病例中显著降低。结论 RP11-307P5.2缺失与喉癌及喉咽癌的发生发展密切相关,未来仍需进一步探明RP11-307P5.

Objective The purpose of this study is to investigate the differential expression of long non-coding RNAs（lnc RNAs）in the carcinoma of larynx and hypopharynx. Methods A systematic literature search was performed,using＂laryngeal/larynx/glottis/supraglottic/subglottic/hypopharyngeal/hypopharynx/laryngopharyngeal/laryngopharynx＂as search term. Only the raw data of affymetrix U133 plus 2.0 arrays was included and imported into the non-coding RNA Function Annotation server（nc FANs）to analyze the dysregulated lnc RNAs. Further,the dysregulated lnc RNAs were validated by q PCR in the tissue samples from our tissue bank. Results Raw data including 17 tumors and 16 normal controls were retrieved and imported into the nc FANs for analysis. Using P value0.001 as cut-off point,there were 1 upregulated and 6 downregulated lnc RNAs in laryngeal and hypopharyngeal carcinoma. Only 1 downregulated lnc RNA（RP11-307 P5.2）was validated to be significantly dysregulated（P〈0.05）in our tissue samples by q PCR. Compared with the early stage cases,the expression of RP11-307 P5.2 was significantly lower in the advanced patients. Conclusion RP11-307 P5.2 was consistently downregulated in laryngeal and hypopharyngeal carcinoma,which could be a potential biomarker or therapeutic target. Further studies on this lnc RNA are needed for elucidating its clinical significance and functional role in laryngeal and hypopharyngeal carcinoma.