凋亡相关蛋白Livin与Caspase-3在弥漫性大B细胞淋巴瘤中的表达及其临床意义

Expression of Apoptosis-related Protein Livin and Caspase-3 in Diffuse Large B Cell Lymphoma and Its Clinical Significance

目的:观察凋亡相关蛋白Livin与Caspase-3在弥漫性大B细胞淋巴瘤(DLBCL)中的表达,探讨其相关性及临床病理意义,为淋巴瘤的临床治疗和预后判断提供参考依据。方法:采用免疫组织化学方法检测51例DLBCL和20例淋巴结反应性增生(RHL)组织中Livin与Caspase-3的表达水平,分析Livin和Caspase-3与DLBCL临床病理特征和预后的关系。结果:51例DLBCL Livin表达率为58.82%(30/51),在RHL中未见表达,(P<0.05)。Livin表达率与DLBCL的临床分期和病理类型相关(P=0.017,P=0.006),与患者性别、年龄及结外受累无关。Caspase-3在51例DLBCL和20例RHL中的表达率分别为25.49%(13/51)和85.0%(17/20)(P<0.05)。Caspase-3的阳性率与DLBCL的临床分期相关(P=0.025)。Caspase-3与患者性别、年龄、病理类型及结外受累无关。Livin与Caspase-3两者在DLBCL的表达呈显著负相关(γs=-0.333,P=0.017)。Kaplan-meier生存分析表明,Livin和Caspase-3的表达与DLBCL患者的预后相关(P=0.0089,P=0.0210)。结论:Livin在DLBCL中高表达,同时在非生发中心亚型的DLBCL中高表达;Caspase-3在DLBCL中低表达,并且两者在DLBCL中表现为负相关。Livin和Caspase-3的表达与DLBCL临床预后相关。Livin与Caspase-3有可能作为判断DLBCL预后的生物学指标。

Objective： To detect the expression levels of Livin and Caspase-3 proteins in diffuse large B cell lymphoma（ DLBCL）,and to explore its correlation with clinicopathological characteristics,so as to provide a reference for clinical treatment and evaluation prognosis of DLBCL. Methods： Immunohisto-chemical staining was performed to detect the expression of Livin and Caspase-3 in the samples from 51 DLBCL patients and 20 patients with reactive hyperplasia of lymph node（ RHL）. The relation between Livin and Caspase-3 with the factors influencing the prognosis of DLBCL was analyzed.Results： Livin＋was not expressed in the tissues of RHL,but its expression rates reached to 58. 82%（ 30/51） in 51 cases of DLBCL（ P〈0. 05）. The expression rats of Caspase-3 in DLBCL and RHL were 25. 49%（ 13/51） and 85. 0%（ 17/20）respectively,and the difference was statistically significant（ P〈0. 05）. The expression level of Livin in DLBCL tissues was related with clinical stage and pathological type（ P〈0. 01,P〈0. 01）. The expression of Livin was not obviously related with sex,age and extranodal involvement. The expression level of Caspase-3 in DLBCL tissues was related with clinical stage（ P〈0. 05）. The expression of Caspase-3 was not obviously related with sex,age,pathological type and extranodal involvement.There was a negative correlation between Livin and Caspase-3 in DLBCL（ γs=-0. 333,P = 0. 017）. Kaplan-meier survival analysis revealed that the expressions of Livin and Caspase-3 were related stalistically significantly with the patients prognosis（ P〈0. 01,P〈0. 05）. Conclusion： Over-expression of Livin in DLBCL and DLBCL of non-GCB subtypes,and lowexpression of Caspase-3 in DLBCL may play a significant role in clinical prognosis of DLBCL.