MicroRNA-15a在儿童原发性免疫性血小板减少症中的的表达及其意义

Expression of MicroRNA-15a in Children with Primary Immune Thrombocytopenia and Its Significance

目的:研究microRNA-15a在儿童原发性免疫性血小板减少症(ITP)中的表达及意义。方法:分离培养ITP患儿及健康志愿者的外周血单个核细胞(PBMNC),应用实时荧光定量PCR法检测miR-15a的表达水平。MiR-15a mimic转染PBMNC后,应用ELISA法检测IFN-γ、IL-2、IL-4、IL-10的含量。结果:在ITP患儿PBMNC中,miR-15a的表达水平显著下降(P<0.05);IFN-γ和IL-2的含量显著上升(P<0.05);IL-4和IL-10的含量显著下降(P<0.05);且miR-15a表达水平与IFN-γ和IL-2含量呈反比(r=-0.793,r=-0.842),与IL-4和IL-10的含量呈正比(r=0.813,r=0.783);过表达miR-15a后可显著抑制IFN-γ和IL-2的产生,促进IL-4和IL-10的生成。结论:miR-15a在ITP患儿PBMNC中的表达显著下降,参与调控Th1/Th2细胞的失衡,提示miR-15a可作为ITP潜在的治疗靶点。

Objective： To analyze the expression of MicroRNA-15a（ miR-15a） in children with primary immune thrombocytopenia（ ITP） and its significance. Methods： The peripheral blood monomuclear cells（ PBMNC） were isolated and cultured from ITP patients and healthy volunteers. The expression level of miR-15a was measured by real-time PCR. After miR-15a mimic was transfected into PBMNC,the levels of INF-γ,IL-2,IL-4 and IL-10 were measured by ELISA. Results：The expression of miR-15a was significantly decreased in PBMNC. The production of IFN-γ and IL-2 was dramatically increased,and the level of IL-4,IL-10 was decreased in PBMNC. Moreover,the expression of miR-15a was negatively correlated with IFN-γ and IL-2,and positively with IL-4 and IL-10. Furthermore,the results showed that the overexpression of miR-15a could decrease the production of IFN-γ and IL-2,and increase the production of IL-4 and IL-10. Conclusion：miR-15a is significantly down-regulated in PBMNC of children with primary ITP and involved in the regulation of Th1/Th2 imbalance. It is suggested that miR-15a may be a potential therapeutic target for ITP.