摘要
背景与目的尿苷二磷酸葡萄糖醛酸转移酶(uridine diphosphate glucuronosyl transferase 1A1,UGT1A1)是伊立替康代谢最主要的同工酶,UGT1A1基因的多态性影响UGT1A1的活性,本研究建立片段分析法检测UGT1A1*28 TATA盒的多态性。方法调取2014年4月-2015年5月在广东省人民医院的住院肺癌患者库存血液标本286例,建立片段分析法检测UGT1A1*28 TATA多态性,与Sanger测序方法比较其精确度和准确度。结果 286例肿瘤血液中,UGT1A1*28 TATA盒TA6/6型有236例(82.5%),TA6/7型有48例(16.8%),TA7/7型2例(0.7%)。片段分析法与Sanger测序法比较,精确度与准确度达100%。结论片段分析法适用于临床检测UGT1A1*28多态性,成本低且方便快捷。
Background and objective Uridine-diphosphoglucuronosyl transferase 1A1 (UGT1A1), UGT1A1*28 polymorphism can reduce UGT1A1 enzymatic activity, which may lead to severe toxicities in patients who receive irinotecan. This study tries to build a fragment analysis method to detect UGTIA1 *28 polymorphism. Methods A total of 286 blood specimens from the lung cancer patients who were hospitalized in Guangdong General Hospital between April 2014 to May 2015 were detected UGTIA1*28 polymorphism by fragment analysis method. Results Comparing with Sanger sequencing, precision and accuracy of the fragment analysis method were 100%. Of the 286 patients, 236 (82.5% harbored TA6/6 genotype, 48 (16.8%) TA 6/7 genotype and 2 (0.7%) TA7/7 genotype. Conclusion Our data suggest hat the fragment analysis method is robust for detecting UGT1A1 *28 polymorphism in clinical practice. It's simple, time-saving, and easy-to-carry.
出处
《中国肺癌杂志》
CAS
CSCD
北大核心
2017年第12期817-821,共5页
Chinese Journal of Lung Cancer
基金
广东省科技厅重点实验室建设项目(No.2012A061400006)资助~~