Linifanib和Tivozanib对肺癌细胞增殖的影响

Effect of Linifanib and Tivozanib on Proliferation of Lung Cancer Cells

为探讨小分子化合物Linifanib和Tivozanib对肺癌细胞A549和H358增殖的影响,以不同浓度Linifanib和Tivozanib预处理细胞48 h,用MTT法检测了细胞存活率,并通过流式细胞仪检测了细胞周期和凋亡情况,用RT-PCR检测了肿瘤抑制因子P21的mRNA表达量.结果表明:Linfanib和Tivozanib能抑制肺癌细胞的增殖,呈现剂量依赖性.Linifanib能诱导细胞凋亡,同时引起细胞周期在G2/M期阻滞.Tivozanib主要引起细胞周期在G2/M期阻滞.Linifanib和Tivozanib均能引起A549和H358细胞中P21的mRNA表达量显著升高,说明Linifanib和Tivozanib能抑制肺癌细胞A549和H358的增殖.

To investigate the effect of Linifanib and Tivozanib on the proliferation of lung cancer cell,A549 and H358 cells were treated with different concentrations of these compounds for 48 h and MTT assay was used to detect cell viability.Cell cycle and cell apoptosis were detected by flow cytometry analysis,and the mRNA expression of tumor-inhibiting factor P21 was tested by RT-PCR.The results showed that Linifanib and Tivozanib could inhibit the proliferation of lung cancer cells in a dose-dependent manner.Linifanib could induce cell apoptosis and arrest cell cycle in G2/M phase.Tivozanib could arrest cell cycle in G2/M phase.Meanwhile P21 mRNA expression in A549 and H358 cells increased after the Linifanib and Tivozanib treatment.It was concluded that Linifanib and Tivozaninb could inhibit the proliferation of lung cancer cell A549 and H358.