白细胞介素32通过5'-LTR抑制HIV复制

Suppression of HIV Replicaton by Interleukin-32 via 5'-LTR

目的:探讨白细胞介素32(IL-32)抑制HIV病毒复制的机制.方法:利用荧光色素酶活性测定筛选抑制HIV复制最显著的IL-32亚型,通过构建HIV的LTR系列截断质粒和TAR茎环结构的系列点突变质粒,确定IL-32抑制HIV复制作用的位点.结果:在Hela、Jurkat、293T三个细胞系中,IL-32γ和IL-32ε两种亚型对HIV的抑制作用最显著.在Hela细胞系中证实IL-32可通过抑制HIV 5'-LTR的活性抑制HIV复制,确定TAR形成的茎环结构在此过程中起重要作用.IL-32与TAR结构相互作用的位点定位在TAR的环状和半环状处.结论:IL-32能抑制HIV复制,可作为HIV治疗的潜在手段.

Objective： To investigate the mechanism of interleukin 32（IL-32） inhibition of HIV replication.Methods：The most significant IL-32 subtype of HIV replication was screened by luciferase activity assays.By constructing a series of HIV plasmids containing LTR truncated mutations and point mutations of TAR hairpin,the site of IL-32 inhibition on HIV replication was determined.Results： In the three cell lines of Hela,Jurkat and 293 T,IL-32γ and IL-32ε subtypes had the most significant inhibitory effects on HIV.In Hela cell line,it was confirmed that IL-32 could suppress HIV replication by inhibiting the activity of HIV 5＇-LTR,with the hairpin structure formed by TAR playing an important role in this process.The action site of IL-32 interaction with TAR structure was located at the ring and the half ring of TAR.Conclusion： IL-32 can inhibit HIV replication as a potential means of HIV treatment.