摘要
将合成DNA的固相亚磷酸酰胺法与自动控制技术相结合,设计了一款基于STM32的96通道寡核苷酸长链的合成仪.该DNA合成仪以STM32F103ZET6微控制器为主控芯片,接收、处理上位机发送的指令,并控制外围的执行单元.人机交互界面采用LabVIEW开发,具有合成序列多通道输入、工作状态监控、与主控系统实时通信等功能.该DNA合成仪控制系统可同时实现96个独立通道的寡核苷酸长链的合成,具有合成效率高、操作界面友好、操作方便等优点,完全满足中小型实验室及社区医院的碱基合成需求.
Combining solid phase phosphoramidite method of DNA synthesis with automatic control technology,a DNA synthesizer of 96 oligonucleotides long chain is designed based on STM32.The core of DNA synthesizer is STM32 F103 ZET6 microcontroller,who is used for receiving and processingrecinstruction from upper computer,and controlling peripheral circuit module.Based on LabVIEW development platform,application software ofupper computer is built,which has function of synthetic sequence multichannel input,working state monitoring and real-time communication with control system.The DNA synthesizer researched in this paper can achieve composition of 96 independent channel oligonucleotides long chain at the same time.It has many advantages,such as high synthetic efficiency,friendly operation interface,and easy operation mode.The DNA synthesizer can fully satisfy requirement of base composition of small and medium-sized laboratory and community hospital.
出处
《中南民族大学学报（自然科学版）》
CAS
北大核心
2017年第4期78-83,共6页
Journal of South-Central University for Nationalities：Natural Science Edition
基金
国家自然科学基金资助项目(81101080)
